The NAD synthetase NadE (OutB) of Bacillus subtilis is a sigma(B)-dependent general stress protein

Autor(en): Antelmann, H
Schmid, R
Hecker, M
Stichwörter: BACTERIAL; DNA; general stress protein; LOCUS; Microbiology; NadE; OutB; RNA; sigma(B); SIGMA-FACTOR
Erscheinungsdatum: 1997
Herausgeber: ELSEVIER SCIENCE BV
Journal: FEMS MICROBIOLOGY LETTERS
Volumen: 153
Ausgabe: 2
Startseite: 405
Seitenende: 409
Zusammenfassung: 
The identification of sigma(B)-dependent general stress proteins is a useful strategy to understand the physiological role of the unspecific stress response in Bacillus subtilis. By N-terminal sequencing of B. subtilis stress proteins Gsp38 was identified as the NAD-synthetase (NadE). NadE was previously characterized as spore outgrowth factor B (OutB) conferring a temperature-sensitive spore outgrowth defective phenotype. Transcriptional studies showed that nadE is strongly induced in response to heat, ethanol and salt stress or after starvation for glucose in a sigma(B)-dependent manner. Two promoters are involved in transcriptional initiation, the sigma(A)-dependent upstream promoter contributes to the basal level during growth, whereas the sigma(B)-dependent downstream promoter is induced after different stress conditions.
ISSN: 03781097
DOI: 10.1016/S0378-1097(97)00281-4

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