ION-CHANNEL PROPERTIES OF THE RECONSTITUTED CHLOROPLAST TRIOSE PHOSPHATE/PHOSPHATE TRANSLOCATOR

Autor(en): SCHWARZ, M
GROSS, A
STEINKAMP, T
FLUGGE, UI
WAGNER, R 
Stichwörter: Biochemistry & Molecular Biology; CARRIER; ENVELOPE; MEMBRANE; MITOCHONDRIAL ASPARTATE GLUTAMATE; PHOSPHATE TRANSLOCATOR; PROTEIN; PURIFICATION; SH-REAGENTS; SPINACH-CHLOROPLASTS; UNIDIRECTIONAL TRANSPORT
Erscheinungsdatum: 1994
Herausgeber: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 269
Ausgabe: 47
Startseite: 29481
Seitenende: 29489
Zusammenfassung: 
The chloroplast triose phosphate/phosphate translocator (cTPT) was isolated from envelope membranes or from transformed yeast cells and reconstituted into artificial membranes. Ionic currents mediated by the cTPT across these membranes were investigated by flux measurements and by the patch clamp technique. The results of the flux measurements indicate that inorganic phosphate (P-i) at saturating concentrations on both sides of the membrane and chloride (Cl-) at all applied concentrations are transported by the cTPT at rates about 20-fold higher than those measured in intact chloroplasts. After reconstitution of the protein into giant liposomes, single channel currents mediated by the cTPT were resolved with the patch-clamp technique. The protein was shown to be a voltage-dependent anion channel with complex gating revealing sublevels with conductances of 12, 54, 96, and 138 pS for Cl- and 6 pS and 18 pS for P-i, respectively. Recordings from patches compromising multiple channels show a synchronously appearing nonlinear current voltage (I/V) relationship in symmetrical buffers, and a different gating at positive and negative membrane potentials. This suggests that the cTPT is incorporated into the membrane in a unidirectional orientation. 3-Phosphoglycerate, a high affinity substrate of the transporter protein, induced a reversible flickering of open channel, and the channel open probability was decreased 60%. It is concluded that, besides its normal counter-exchange mode, the cTPT can also work as a voltage-dependent anion selective channel.
ISSN: 00219258

Show full item record

Page view(s)

1
Last Week
1
Last month
0
checked on Feb 27, 2024

Google ScholarTM

Check