Design and synthesis of Raman reporter molecules for tissue imaging by immuno-SERS microscopy

Autor(en): Schuetz, Max
Mueller, Christian I.
Salehi, Mohammad
Lambert, Christoph
Schluecker, Sebastian
Stichwörter: ANTIGEN; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; CHROMOPHORES; LABELS; NANOPARTICLE PROBES; nanoshell; Optics; QUANTUM DOTS; Raman reporter; SCATTERING; SELF-ASSEMBLED MONOLAYERS; SERS; tissue imaging; VALENCE
Erscheinungsdatum: 2011
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: JOURNAL OF BIOPHOTONICS
Volumen: 4
Ausgabe: 6, SI
Startseite: 453
Seitenende: 463
Zusammenfassung: 
The design and synthesis of Raman reporter molecules comprising olefin or alkyne moieties with strong and characteristic vibrational Raman bands is presented. Chemisorption onto the surface of colloidal Au/Ag shells yields a self-assembled monolayer. Hydrophilic stabilization of such SERS labels can be achieved by short terminal ethylene glycol units attached to the Raman reporter. Encapsulation by silica with subsequent functionalization of the glass surface allows the conjugation to biomolecules such as antibodies. We demonstrate the use of SERS-labeled antibodies for tissue imaging of the tumor suppressor p63 in prostate biopsies. [GRAPHICS] Specifically designed Raman reporter molecules form a self-assembled monolayer on the surface of Au/Ag nanoshells. Such SERS labels can be used for tissue imaging after conjugation to target-specific ligands such as antibodies.
ISSN: 1864063X
DOI: 10.1002/jbio.201000116

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