OEP37 is a new member of the chloroplast outer membrane ion channels

DC FieldValueLanguage
dc.contributor.authorGoetze, Tom Alexander
dc.contributor.authorPhilippar, Katrin
dc.contributor.authorIlkavets, Irina
dc.contributor.authorSoll, Jurgen
dc.contributor.authorWagner, Richard
dc.date.accessioned2021-12-23T16:12:10Z-
dc.date.available2021-12-23T16:12:10Z-
dc.date.issued2006
dc.identifier.issn00219258
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10073-
dc.description.abstractThe chloroplast outer envelope protein OEP37 is a member of the growing beta-barrel protein family of the outer chloroplast membrane. The reconstituted recombinant protein OEP37 from pea forms a rectifying high conductance channel with a main conductance (lambda) of Lambda = 500 picosiemens (symmetrical 250 mM KCl). The OEP37 channel is cation-selective (PK+/P-Cl- = 14:1) with a voltage-dependent open probability maximal at V-mem = 0 mV. The channel pore reveals an hourglass-shaped form with different diameters for the vestibule and restriction zone. The diameters of the vestibule at the high conductance side were estimated by d = 3.0 nm and the restriction zone by d = 1.5 nm. The OEP37 channel displayed a nanomolar affinity for the precursor of the chloroplast inner membrane protein Tic32, which is imported into the chloroplast through a yet unknown pathway. Pre-proteins imported through the usual Toc pathway and synthetic control peptides, however, did not show a comparable block of the OEP37 channel. In addition to the electrophysiological characterization, we studied the gene expression of OEP37 in the model plant Arabidopsis thaliana. Here, transcripts of AtOEP37 are ubiquitously expressed throughout plant development and accumulate in early germinating seedlings as well as in late embryogenesis. The plastid intrinsic protein could be detected in isolated chloroplasts of cotyledons and rosette leaves. However, the knock-out mutant oep37-1 shows that the proper function of this single copy gene is not essential for development of the mature plant. Moreover, import of Tic32 into chloroplasts of oep37-1 was not impaired when compared with wild type. Thus, OEP37 may constitute a novel peptide-sensitive ion channel in the outer envelope of plastids with function during embryogenesis and germination.
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.relation.ispartofJOURNAL OF BIOLOGICAL CHEMISTRY
dc.subjectBiochemistry & Molecular Biology
dc.subjectCONDUCTANCE
dc.subjectENVELOPE
dc.subjectK+-CHANNEL
dc.subjectPORE
dc.subjectPREPROTEIN TRANSLOCASE
dc.subjectPROTEIN IMPORT
dc.subjectSELECTIVITY
dc.subjectSOLUTE CHANNEL
dc.subjectVDAC
dc.titleOEP37 is a new member of the chloroplast outer membrane ion channels
dc.typejournal article
dc.identifier.doi10.1074/jbc.M600700200
dc.identifier.isiISI:000238490300049
dc.description.volume281
dc.description.issue26
dc.description.startpage17989
dc.description.endpage17998
dc.contributor.orcid0000-0002-2140-4907
dc.contributor.researcheridF-7942-2018
dc.contributor.researcheridI-1891-2013
dc.identifier.eissn1083351X
dc.publisher.place11200 ROCKVILLE PIKE, SUITE 302, ROCKVILLE, MD, UNITED STATES
dcterms.isPartOf.abbreviationJ. Biol. Chem.
dcterms.oaStatushybrid
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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