Characterization of a proteasome alpha-chain from Giardia lamblia

DC FieldValueLanguage
dc.contributor.authorEmmerlich, V
dc.contributor.authorScholze, H
dc.contributor.authorGillin, FD
dc.contributor.authorBakker-Grunwald, T
dc.date.accessioned2021-12-23T16:12:15Z-
dc.date.available2021-12-23T16:12:15Z-
dc.date.issued2001
dc.identifier.issn09320113
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10126-
dc.description.abstractTo begin to characterize the components of the 20S proteasome of Giardia lamblia, we have cloned a genomic sequence encoding an alpha -chain (type alpha3/C9, predicted size 244 amino acid residues). Southern analysis indicated that a single gene codes for this protein, and a Northern blot exhibited a single signal at 850 nt. An antiserum against a C-terminal fragment of the alpha -chain expressed in Escherichia coli reacted with a single protein band of M-r 27,000 that was present at constant levels in trophozoites and encysting cells. On a 2D blot of the purified 20S proteasome, we identified the crossreacting component as a single protein of IEP 6.0, in agreement with the IEP predicted by the coding sequence. Our data confirm that the G. lamblia 20S proteasome is typically eukaryotic in containing a set of diverged alpha -subunits.
dc.description.sponsorshipNATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI042488] Funding Source: NIH RePORTER; NIAID NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI42488] Funding Source: Medline
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPARASITOLOGY RESEARCH
dc.subjectGENE
dc.subjectParasitology
dc.subjectRNA
dc.titleCharacterization of a proteasome alpha-chain from Giardia lamblia
dc.typejournal article
dc.identifier.doi10.1007/s004360000332
dc.identifier.isiISI:000166482400004
dc.description.volume87
dc.description.issue2
dc.description.startpage112
dc.description.endpage115
dc.identifier.eissn14321955
dc.publisher.placeONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES
dcterms.isPartOf.abbreviationParasitol. Res.
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