Binding of interferon reduces the force of unfolding for interferon receptor 1
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Chuartzman, Silvia G. | |
dc.contributor.author | Nevo, Reinat | |
dc.contributor.author | Waichman, Sharon | |
dc.contributor.author | Shental, Dalit | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Levy, Yaakov | |
dc.contributor.author | Reich, Ziv | |
dc.contributor.author | Kapon, Ruti | |
dc.date.accessioned | 2021-12-23T16:12:18Z | - |
dc.date.available | 2021-12-23T16:12:18Z | - |
dc.date.issued | 2017 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10143 | - |
dc.description.abstract | Differential signaling of the type I interferon receptor (IFNAR) has been correlated with the ability of its subunit, IFNAR1, to differentially recognize a large spectrum of different ligands, which involves intricate conformational re-arrangements of multiple interacting domains. To shed light onto the structural determinants governing ligand recognition, we compared the force-induced unfolding of the IFNAR1 ectodomain when bound to interferon and when free, using the atomic force microscope and steered molecular dynamics simulations. Unexpectedly, we find that IFNAR1 is easier to mechanically unfold when bound to interferon than when free. Analysis of the structures indicated that the origin of the reduction in unfolding forces is a conformational change in IFNAR1 induced by ligand binding. | |
dc.description.sponsorship | ERA-NET NanoScience program; DFGGerman Research Foundation (DFG)European Commission [PI 405/4]; ISFIsrael Science Foundation [2135/06-88.0]; JP and RK recieved funding from the ERA-NET NanoScience program, DFG grant no. PI 405/4, ISF grant no. 2135/06-88.0 https://ec.europa.eu/research/fp7/index_en.cfm?pg=eranet-projects-home) . The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.language.iso | en | |
dc.publisher | PUBLIC LIBRARY SCIENCE | |
dc.relation.ispartof | PLOS ONE | |
dc.subject | ACTIVATION | |
dc.subject | CYTOKINE | |
dc.subject | I INTERFERONS | |
dc.subject | IFN-ALPHA-2 | |
dc.subject | IFNAR1 | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | MUTATIONAL ANALYSIS | |
dc.subject | PHAGE DISPLAY | |
dc.subject | PROTEIN | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SPECTROSCOPY | |
dc.subject | STRUCTURAL BASIS | |
dc.title | Binding of interferon reduces the force of unfolding for interferon receptor 1 | |
dc.type | journal article | |
dc.identifier.doi | 10.1371/journal.pone.0175413 | |
dc.identifier.isi | ISI:000399955200063 | |
dc.description.volume | 12 | |
dc.description.issue | 4 | |
dc.publisher.place | 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA | |
dcterms.isPartOf.abbreviation | PLoS One | |
dcterms.oaStatus | Green Submitted, Green Published, gold | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 15.05.2024