Diffraction-Unlimited Photomanipulation at the Plasma Membrane via Specifically Targeted Upconversion Nanoparticles
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Drees, Christoph | |
dc.contributor.author | Ruehl, Philipp | |
dc.contributor.author | Czerny, Jacqueline | |
dc.contributor.author | Chandra, Gemini | |
dc.contributor.author | Bajorath, Janosch | |
dc.contributor.author | Haase, Markus | |
dc.contributor.author | Heinemann, Stefan H. | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:12:22Z | - |
dc.date.available | 2021-12-23T16:12:22Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10184 | - |
dc.description.abstract | Engineered UCNP are used to trigger rapid photoconversion of the fluorescent protein Dendra2 with nanoscopic precision and over longer distances in mammalian cells. By exploiting the synergy of high-level thulium doping with core-shell design and elevated excitation intensities, intense UCNP emission is achieved, allowing fast photoconversion of Dendra2 with <10 nm resolution. A tailored biocompatible surface coating and functionalization with a derivate of green fluorescent protein (GFP) for recognition of antiGFP nanobodies are developed. Highly specific targeting of UCNP to fusion proteins of antiGFP on the surface of mammalian cells is demonstrated. UCNP bound to extracellular Dendra2 enable rapid photoconversion selectively in molecular proximity and thus unambiguous detection of cytokine receptor dimerization in the plasma membrane and in endosomes. Remarkably, UCNPs are for suited for manipulating intracellular Dendra2 across the plasma membrane. This study thus establishes UCNP-controlled photomanipulation with nanoscale precision, opening exciting opportunities for bioanalytical applications in cell biology. | |
dc.description.sponsorship | Osnabruck University; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [HE 2993/161, PI 405/14-1]; Deutscher Akademischer AustauschdienstDeutscher Akademischer Austausch Dienst (DAAD); Abbe Center of Photonics; We thank G. Hikade, H. Kenneweg, K. Rucker, L. Birk, R. Kurre, K. Cosentino, W. Paa, F. Garwe, and F. Henschel for technical support as well as C. You and C. P. Richter for helpful advice. This project was supported by intramural funding from Osnabruck University (Profile line ``Integrated Science'', M.H. and J.P.), the Deutsche Forschungsgemeinschaft (HE 2993/161, S.H.H., and iBiOs facility PI 405/14-1, J.P.), the Deutscher Akademischer Austauschdienst (G.C.), and the Abbe Center of Photonics (S.H.H.). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | BIOSENSOR | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | cytokine receptor | |
dc.subject | energy transfer | |
dc.subject | GREEN FLUORESCENT PROTEIN | |
dc.subject | LANTHANIDE | |
dc.subject | LIGHT | |
dc.subject | LUMINESCENCE | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | nanoparticles | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | OPTOGENETICS | |
dc.subject | photoactivation | |
dc.subject | PHOTODYNAMIC THERAPY | |
dc.subject | photomanipulation | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | RESONANCE ENERGY-TRANSFER | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | ULTRASENSITIVE DETECTION | |
dc.subject | upconversion | |
dc.subject | UPCONVERTING NANOPARTICLES | |
dc.title | Diffraction-Unlimited Photomanipulation at the Plasma Membrane via Specifically Targeted Upconversion Nanoparticles | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/acs.nanolett.1c02267 | |
dc.identifier.isi | ISI:000709549100016 | |
dc.description.volume | 21 | |
dc.description.issue | 19 | |
dc.description.startpage | 8025 | |
dc.description.endpage | 8034 | |
dc.contributor.orcid | 0000-0001-6083-3205 | |
dc.contributor.orcid | 0000-0002-9686-8810 | |
dc.contributor.orcid | 0000-0002-3554-3107 | |
dc.identifier.eissn | 15306992 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-9686-8810 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | HaMa954 | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 10.05.2024