Strain-specific differences in pili formation and the interaction of Corynebacterium diphtheriae with host cells

DC FieldValueLanguage
dc.contributor.authorOtt, Lisa
dc.contributor.authorHoeller, Martina
dc.contributor.authorRheinlaender, Johannes
dc.contributor.authorSchaeffer, Tilman E.
dc.contributor.authorHensel, Michael
dc.contributor.authorBurkovski, Andreas
dc.date.accessioned2021-12-23T16:12:31Z-
dc.date.available2021-12-23T16:12:31Z-
dc.date.issued2010
dc.identifier.issn14712180
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10257-
dc.description.abstractBackground: Corynebacterium diphtheriae, the causative agent of diphtheria, is well-investigated in respect to toxin production, while little is known about C. diphtheriae factors crucial for colonization of the host. In this study, we investigated strain-specific differences in adhesion, invasion and intracellular survival and analyzed formation of pili in different isolates. Results: Adhesion of different C. diphtheriae strains to epithelial cells and invasion of these cells are not strictly coupled processes. Using ultrastructure analyses by atomic force microscopy, significant differences in macromolecular surface structures were found between the investigated C. diphtheriae strains in respect to number and length of pili. Interestingly, adhesion and pili formation are not coupled processes and also no correlation between invasion and pili formation was found. Using RNA hybridization and Western blotting experiments, strain-specific pili expression patterns were observed. None of the studied C. diphtheriae strains had a dramatic detrimental effect on host cell viability as indicated by measurements of transepithelial resistance of Detroit 562 cell monolayers and fluorescence microscopy, leading to the assumption that C. diphtheriae strains might use epithelial cells as an environmental niche supplying protection against antibodies and macrophages. Conclusions: The results obtained suggest that it is necessary to investigate various isolates on a molecular level to understand and to predict the colonization process of different C. diphtheriae strains.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 796, B5, C1]; The authors wish to thank C. von Hunolstein (Istituto Superiore di Sanita, Rome) for providing strain ISS3319, ISS4060, ISS4746, and ISS4749, as well as H. Ton-That (University of Texas Health Science Center, Houston, TX) for SpaD antibodies, SpaD protein, and chromosomal DNA of strain NCTC13129. The help of R. G. Gerlach (Mikrobiologisches Institut des Universitatsklinikums Erlangen) to establish the epithelial resistance assay is gratefully acknowledged. This study was financially supported by the Deutsche Forschungsgemeinschaft for in frame of SFB 796 (projects B5, C1, and Z).
dc.language.isoen
dc.publisherBMC
dc.relation.ispartofBMC MICROBIOLOGY
dc.subjectADHERENCE
dc.subjectADHESION
dc.subjectEMERGENCE
dc.subjectEPIDEMIOLOGY
dc.subjectINTERNALIZATION
dc.subjectMicrobiology
dc.subjectMITIS
dc.subjectORGANIZATION
dc.subjectPATHOGENICITY
dc.subjectPROTEINS
dc.subjectSURFACE
dc.titleStrain-specific differences in pili formation and the interaction of Corynebacterium diphtheriae with host cells
dc.typejournal article
dc.identifier.doi10.1186/1471-2180-10-257
dc.identifier.isiISI:000283556600001
dc.description.volume10
dc.contributor.orcid0000-0001-5643-8384
dc.contributor.orcid0000-0002-1976-9245
dc.contributor.orcid0000-0003-1896-4521
dc.contributor.orcid0000-0002-9779-9052
dc.contributor.researcheridQ-7995-2017
dc.publisher.placeCAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
dcterms.isPartOf.abbreviationBMC Microbiol.
dcterms.oaStatusGreen Published, gold
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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