Stable Hairpin Structures Formed by Xylose-Based Nucleic Acids

Autor(en): Mattelaer, Charles-Alexandre
Maiti, Mohitosh
Smets, Laurent
Maiti, Munmun
Schepers, Guy
Mattelaer, Henri-Philippe
Rosemeyer, Helmut 
Herdewijn, Piet
Lescrinier, Eveline
Stichwörter: aptamers; Biochemistry & Molecular Biology; Chemistry, Medicinal; molecular dynamics; NMR spectroscopy; Pharmacology & Pharmacy; structural elucidation; XNA
Erscheinungsdatum: 2021
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: CHEMBIOCHEM
Volumen: 22
Ausgabe: 9
Startseite: 1638
Seitenende: 1645
Zusammenfassung: 
Xenobiology explores synthetic nucleic acid polymers as alternative carriers of genetic information to expand the central dogma. The xylo- and deoxyxylo-nucleic acids (XyNA and dXyNA), containing 3' epimers of riboses and deoxyriboses, are considered to be potential candidates for an orthogonal system. In this study, thermal and spectroscopic analyses show that XyNA and dXyNA form stable hairpins. The dXyNA hairpin structure determined by NMR spectroscopy contains a flexible loop that locks the stem into a stable ladder-like duplex with marginal right-handed helicity. The reduced flexibility of the dXyNA duplex observed in the stem of the hairpin demonstrates that folding of dXyNA yields more stable structures described so far.
ISSN: 14394227
DOI: 10.1002/cbic.202000803

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