AP-3 vesicle uncoating occurs after HOPS-dependent vacuole tethering
DC Element | Wert | Sprache |
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dc.contributor.author | Schoppe, Jannis | |
dc.contributor.author | Mari, Muriel | |
dc.contributor.author | Yavavli, Erdal | |
dc.contributor.author | Auffarth, Kathrin | |
dc.contributor.author | Cabrera, Margarita | |
dc.contributor.author | Walter, Stefan | |
dc.contributor.author | Froehlich, Florian | |
dc.contributor.author | Ungermann, Christian | |
dc.date.accessioned | 2021-12-23T16:12:47Z | - |
dc.date.available | 2021-12-23T16:12:47Z | - |
dc.date.issued | 2020 | |
dc.identifier.issn | 02614189 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10282 | - |
dc.description.abstract | Heterotetrameric adapter (AP) complexes cooperate with the smallGTPase Arf1 or lipids in cargo selection, vesicle formation, and budding at endomembranes in eukaryotic cells. While mostAPcomplexes also require clathrin as the outer vesicle shell, formation ofAP-3-coated vesicles involved in Golgi-to-vacuole transport in yeast has been postulated to depend on Vps41, a subunit of the vacuolarHOPStethering complex.HOPShas also been identified as the tether ofAP-3 vesicles on vacuoles. To unravel this conundrum of a dual Vps41 function, we anchored Vps41 stably to the mitochondrial outer membrane. By monitoringAP-3 recruitment, we now show that Vps41 can tetherAP-3 vesicles to mitochondria, yetAP-3 vesicles can form in the absence of Vps41 or clathrin. By proximity labeling and mass spectrometry, we identify the Arf1GTPase-activating protein (GAP) Age2 at theAP-3 coat and show that tethering, but not fusion at the vacuole can occur without complete uncoating. We conclude thatAP-3 vesicles retain their coat after budding and that their complete uncoating occurs only after tethering at the vacuole. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [SFB 944]; NWO open program [ALW.355]; Projekt DEAL; We thank Kathrin Auffarth and Angela Perz for expert technical assistance. This work was supported by grants of the DFG (SFB 944, project P11 to C.U., and P20 to F.F.) and the NWO open program ALW.355 (M.M.). Open access funding enabled and organized by Projekt DEAL. | |
dc.language.iso | en | |
dc.publisher | WILEY | |
dc.relation.ispartof | EMBO JOURNAL | |
dc.subject | ADAPTER PROTEIN COMPLEX | |
dc.subject | ALKALINE-PHOSPHATASE | |
dc.subject | AP-3 | |
dc.subject | ARF FAMILY | |
dc.subject | Arf1 GAP | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Cell Biology | |
dc.subject | FUSION | |
dc.subject | GOLGI | |
dc.subject | HOPS | |
dc.subject | MEMBRANE-TRANSPORT | |
dc.subject | RAB INTERACTIONS | |
dc.subject | RECRUITMENT | |
dc.subject | SELECTIVE TRANSPORT | |
dc.subject | vacuole | |
dc.subject | Vps41 | |
dc.subject | YEAST R-SNARE | |
dc.title | AP-3 vesicle uncoating occurs after HOPS-dependent vacuole tethering | |
dc.type | journal article | |
dc.identifier.doi | 10.15252/embj.2020105117 | |
dc.identifier.isi | ISI:000562236100001 | |
dc.description.volume | 39 | |
dc.description.issue | 20 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.contributor.orcid | 0000-0002-2677-6945 | |
dc.contributor.orcid | 0000-0002-2978-8255 | |
dc.contributor.researcherid | F-3306-2016 | |
dc.identifier.eissn | 14602075 | |
dc.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | |
dcterms.isPartOf.abbreviation | Embo J. | |
dcterms.oaStatus | hybrid, Green Published | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | FrFl166 | - |
crisitem.author.netid | UnCh999 | - |
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geprüft am 14.05.2024