Identification of a Glucose Permease from Mycobacterium smegmatis mc(2) 155
Autor(en): | Pimentel-Schmitt, Elisngela F. Jahreis, Knut Eddy, Mike P. Amon, Johannes Burkovski, Andreas Titgemeyer, Fritz |
Stichwörter: | Bacterial lifestyle; Biotechnology & Applied Microbiology; Carbon regulation; ESCHERICHIA-COLI; FUNCTIONAL EXPRESSION; LOCALIZATION; Membrane topology; Microbiology; Mycobacterium; Pathogenicity; PHOSPHOTRANSFERASE SYSTEM; PROTEIN; REPRESSION; SEQUENCE; SKELETAL-MUSCLE; STREPTOMYCES-COELICOLOR A3(2); Sugar transport; SUGAR-TRANSPORT | Erscheinungsdatum: | 2009 | Herausgeber: | KARGER | Enthalten in: | JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY | Band: | 16 | Ausgabe: | 3-4 | Startseite: | 169 | Seitenende: | 175 | Zusammenfassung: | We report here the molecular identification of a glucose permease from Mycobacterium smegmatis, a model organism for our understanding of the life patterns of the major pathogens Mycobacterium tuberculosis and Mycobacterium leprae. A computer-based search of the available genome of M. smegmatis mc(2) 155 with the sequences of well-characterized glucose transporters revealed the gene msmeg4187 as a possible candidate. The deduced protein belongs to the major facilitator superfamily of proton symporters and facilitators and exhibits up to 53% of amino acid identity to other members of this family. Heterologous expression of msmeg4187 in an Escherichia coli glucose-negative mutant led to the restoration of growth on glucose. The determination of the biochemical features characterize MSMEG4187 (GlcP) as a high affinity (K m of 19 mu M), glucose-specific permease. The results represent the first molecular characterization of a sugar permease in mycobacteria, and thus supply fundamental data for further in-depth analysis on the nutritional lifestyle of these bacteria. Copyright (C) 2008 S. Karger AG, Basel |
ISSN: | 14641801 | DOI: | 10.1159/000119546 |
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geprüft am 09.06.2024