IDENTIFICATION OF A METHYL-SPECIFIC RESTRICTION SYSTEM MEDIATED BY A CONJUGATIVE ELEMENT FROM STREPTOMYCES-BAMBERGIENSIS

DC ElementWertSprache
dc.contributor.authorZOTCHEV, SB
dc.contributor.authorSCHREMPF, H
dc.contributor.authorHUTCHINSON, CR
dc.date.accessioned2021-12-23T16:13:08Z-
dc.date.available2021-12-23T16:13:08Z-
dc.date.issued1995
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10417-
dc.description.abstractpBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor, pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome of S. bambergiensis. S. lividans carrying pBL2 or pBL2.1 acquired a methyl specific restriction (MsrA(+)) phenotype. The corresponding enzyme was partially purified and shown to resemble a class II endonuclease which cleaves Dam-methylated DNA preferentially.
dc.description.sponsorshipNATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM031925] Funding Source: NIH RePORTER; NIGMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [GM 31925] Funding Source: Medline
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectCLONING
dc.subjectDNA RESTRICTION
dc.subjectESCHERICHIA-COLI
dc.subjectMicrobiology
dc.titleIDENTIFICATION OF A METHYL-SPECIFIC RESTRICTION SYSTEM MEDIATED BY A CONJUGATIVE ELEMENT FROM STREPTOMYCES-BAMBERGIENSIS
dc.typenote
dc.identifier.doi10.1128/jb.177.16.4809-4812.1995
dc.identifier.isiISI:A1995RN69600030
dc.description.volume177
dc.description.issue16
dc.description.startpage4809
dc.description.endpage4812
dc.contributor.orcid0000-0002-9324-245X
dc.identifier.eissn10985530
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScHi752-
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