A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Gropeanu, Mihaela | |
dc.contributor.author | Bhagawati, Maniraj | |
dc.contributor.author | Gropeanu, Radu A. | |
dc.contributor.author | Muniz, Gemma M. Rodriguez | |
dc.contributor.author | Sundaram, Subramanian | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | del Campo, Aranzazu | |
dc.date.accessioned | 2021-12-23T16:13:23Z | - |
dc.date.available | 2021-12-23T16:13:23Z | - |
dc.date.issued | 2013 | |
dc.identifier.issn | 16136810 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10542 | - |
dc.description.abstract | Photocleavable oligohistidine peptides (POHP) allow in situ spatial organization of multiple His-tagged proteins onto surfaces functionalized with tris(nitrilotriacetic acid) (tris-NTA). Here, a second generation of POHPs is presented with improved photoresponse and site-specific covalent coupling is introduced for generating stable protein assemblies. POHPs with different numbers of histidine residues and a photocleavable linker based on the 4,5-dimethoxy-o-nitrophenyl ethyl chromophore are prepared. These peptides show better photosensitivity than the previously used o-nitrophenyl ethyl derivative. Efficient and stable caging of tris-NTA-functionalized surfaces by POHPs comprising 12 histidine residues is demonstrated by multiparameter solid-phase detection techniques. Laser lithographic uncaging by photofragmentation of the POHPs is possible with substantially reduced photodamage as compared to previous approaches. Thus, in situ micropatterning of His-tagged proteins under physiological conditions is demonstrated for the first time. In combination with a short peptide tag for a site-specific enzymatic coupling reaction, covalent immobilization of multiple proteins into target micropatterns is possible under physiological conditions. | |
dc.description.sponsorship | BMBFFederal Ministry of Education & Research (BMBF) [0312005A]; DFGGerman Research Foundation (DFG)European Commission [CA880/3-1]; M.G. and M.B. contributed equally to this work. Authors thank Dr. Veronica San Miguel and Catarina Custodio (Max-Planck-Institut fur Polymerforschung) for comparative photolysis experiments on the chromophores, and Marta Rodriguez for assistance with Rlf measurements. A.d.C. and J.P. acknowledge financial support from the BMBF (0312005A). A.d.C acknowledges financial support from the DFG (CA880/3-1). | |
dc.language.iso | en | |
dc.publisher | WILEY-V C H VERLAG GMBH | |
dc.relation.ispartof | SMALL | |
dc.subject | BIOMOLECULES | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | FABRICATION | |
dc.subject | FUNCTIONAL IMMOBILIZATION | |
dc.subject | GLASS | |
dc.subject | histidine tags | |
dc.subject | HISTIDINE-TAGGED PROTEINS | |
dc.subject | laser lithography | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MOTOR PROTEINS | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | photocleavable peptides | |
dc.subject | PHOTOLABILE PROTECTING GROUPS | |
dc.subject | phototriggers | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | protein micropatterns | |
dc.subject | RECEPTOR INTERACTIONS | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SURFACE | |
dc.subject | THIOLS | |
dc.title | A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/smll.201201901 | |
dc.identifier.isi | ISI:000316338800006 | |
dc.description.volume | 9 | |
dc.description.issue | 6 | |
dc.description.startpage | 838 | |
dc.description.endpage | 845 | |
dc.contributor.orcid | 0000-0001-5725-2135 | |
dc.identifier.eissn | 16136829 | |
dc.publisher.place | POSTFACH 101161, 69451 WEINHEIM, GERMANY | |
dcterms.isPartOf.abbreviation | Small | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 13.05.2024