Establishing a model to study the regulation of the lactose operon in Lactobacillus casei

Autor(en): Gosalbes, MJ
Monedero, V
Alpert, CA
PerezMartinez, G
Stichwörter: CATABOLITE REPRESSION; DNA; GENE; LACTIC-ACID BACTERIA; Lactobacillus casei; lactose operon; Microbiology; MOLECULAR-CLONING; NUCLEOTIDE-SEQUENCE; PHOSPHOENOLPYRUVATE; PHOSPHOTRANSFERASE SYSTEM; RNA; transcriptional antitermination
Erscheinungsdatum: 1997
Herausgeber: ELSEVIER SCIENCE BV
Journal: FEMS MICROBIOLOGY LETTERS
Volumen: 148
Ausgabe: 1
Startseite: 83
Seitenende: 89
Zusammenfassung: 
The chromosomally encoded lactose-specific phosphoenol pyruvate-dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15(-)] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15(-)] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.
ISSN: 03781097
DOI: 10.1016/S0378-1097(97)00017-7

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