Archaeal transcriptional regulation of the prokaryotic KdpFABC complex mediating K+ uptake in H. salinarum

DC FieldValueLanguage
dc.contributor.authorKixmueller, Dorthe
dc.contributor.authorStrahl, Henrik
dc.contributor.authorWende, Andy
dc.contributor.authorGreie, Joerg-Christian
dc.date.accessioned2021-12-23T16:13:34Z-
dc.date.available2021-12-23T16:13:34Z-
dc.date.issued2011
dc.identifier.issn14310651
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10631-
dc.description.abstractThe genome of the halophilic archaeon Halobacterium salinarum encodes the high-affinity ATP-dependent K+ uptake system Kdp. Previous studies have shown that the genes coding for the KdpFABC complex are arranged in a kdpFABCQ gene cluster together with an additional gene kdpQ. In bacteria, expression of the kdpFABC genes is generally regulated by the dedicated sensor kinase/response regulator pair KdpD/KdpE, which are absent in H. salinarum. Surprisingly, H. salinarum expresses the kdp genes in a manner which is strikingly similar to Escherichia coli. In this study, we show that the halobacterial kdpFABCQ genes constitute an operon and that kdpFABCQ expression is subject to a complex regulatory mechanism involving a negative transcriptional regulator and is further modulated via a so far unknown mechanism. We describe how the regulation of kdp gene expression is facilitated in H. salinarum in contrast to its bacterial counterparts. Whereas the Kdp system fulfils the same core function as an ATP-driven K+ uptake system in both archaea and bacteria, the different mechanisms involved in the regulation of gene expression appear to have evolved separately, possibly reflecting a different physiological role of ATP-driven K+ uptake in halophilic archaea.
dc.description.sponsorshipBoehringer Ingelheim FondsBoehringer Ingelheim; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [GR 2698/1-1]; Ursula Krehe is acknowledged for technical assistance. Further, we thank Ertan Ozyamak for construction of plasmid pDSK1. H. S. was supported by the Boehringer Ingelheim Fonds. This work was supported by the Deutsche Forschungsgemeinschaft (grant GR 2698/1-1).
dc.language.isoen
dc.publisherSPRINGER TOKYO
dc.relation.ispartofEXTREMOPHILES
dc.subjectBiochemistry & Molecular Biology
dc.subjectCOMPLETE GENOME SEQUENCE
dc.subjectESCHERICHIA-COLI K-12
dc.subjectEXPRESSION
dc.subjectGene regulation
dc.subjectHaloarchaea
dc.subjectHalobacterium
dc.subjectHALOBACTERIUM-SALINARUM
dc.subjectHALOFERAX-ALICANTEI
dc.subjectHALOPHILIC BETA-GALACTOSIDASE
dc.subjectKdpD
dc.subjectKdpFABC
dc.subjectKdpQ
dc.subjectMicrobiology
dc.subjectPotassium uptake
dc.subjectPROMOTER
dc.subjectSENSOR KINASE KDPD
dc.subjectSTRAIN R1
dc.subjectSULFOLOBUS-SOLFATARICUS
dc.titleArchaeal transcriptional regulation of the prokaryotic KdpFABC complex mediating K+ uptake in H. salinarum
dc.typejournal article
dc.identifier.doi10.1007/s00792-011-0395-y
dc.identifier.isiISI:000296011800003
dc.description.volume15
dc.description.issue6
dc.description.startpage643
dc.description.endpage652
dc.contributor.orcid0000-0003-4416-2178
dc.publisher.place1-11-11 KUDAN-KITA, CHIYODA-KU, TOKYO, 102-0073, JAPAN
dcterms.isPartOf.abbreviationExtremophiles
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