Nanoscale Organization of Mitochondrial Microcompartments Revealed by Combining Tracking and Localization Microscopy

DC FieldValueLanguage
dc.contributor.authorAppelhans, Timo
dc.contributor.authorRichter, Christian P.
dc.contributor.authorWilkens, Verena
dc.contributor.authorHess, Samuel T.
dc.contributor.authorPiehler, Jacob
dc.contributor.authorBusch, Karin B.
dc.date.accessioned2021-12-23T16:13:41Z-
dc.date.available2021-12-23T16:13:41Z-
dc.date.issued2012
dc.identifier.issn15306984
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10697-
dc.description.abstractWhile detailed information on the nanoscale-organization of proteins within intracellular membranes has emerged from electron microcopy, information on their spatiotemporal dynamics is scarce. By use of a photostable rhodamine attached specifically to Halo-tagged proteins in mitochondrial membranes, we were able to track and localize single protein complexes such as Tom20 and ATP synthase in suborganellar structures in live cells. Individual membrane proteins in the inner and outer membrane of mitochondria were imaged over long time periods with localization precisions below 15 nm. Projection of single molecule trajectories revealed diffusion-restricting rnicrocompartments such as individual cristae in mitochondria. At the same time, protein-specific diffusion characteristics within different mitochondrial membranes could be extracted.
dc.description.sponsorshipDFGGerman Research Foundation (DFG)European Commission [BU2288/1-1, SFB 944]; We thank Wladislaw Kohl and Markus Staufenbiel for excellent technical assistance. It is thankfully acknowledged that complex I subunit 30kD template was obtained from Ulrich Brandt, paGFP from Jennifer Lippincott-Schwarz, and hFis1 from Black Hill. This project was supported by the DFG (BU2288/1-1 and SFB 944).
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofNANO LETTERS
dc.subjectATP synthase
dc.subjectCELLS
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Physical
dc.subjectDYNAMICS
dc.subjectINNER MEMBRANE
dc.subjectMaterials Science
dc.subjectMaterials Science, Multidisciplinary
dc.subjectmembrane protein dynamics
dc.subjectmitochondria
dc.subjectMOLECULES
dc.subjectNanoscience & Nanotechnology
dc.subjectOPTICAL RECONSTRUCTION MICROSCOPY
dc.subjectPARTICLE-TRACKING
dc.subjectPhysics
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectPROTEINS
dc.subjectScience & Technology - Other Topics
dc.subjectSINGLE
dc.subjectsingle molecule tracking
dc.subjectSuper-resolution imaging
dc.subjectTALM
dc.subjectTom20
dc.titleNanoscale Organization of Mitochondrial Microcompartments Revealed by Combining Tracking and Localization Microscopy
dc.typejournal article
dc.identifier.doi10.1021/nl203343a
dc.identifier.isiISI:000299967800014
dc.description.volume12
dc.description.issue2
dc.description.startpage610
dc.description.endpage616
dc.contributor.orcid0000-0003-0525-0191
dc.contributor.researcheridAAM-8374-2021
dc.contributor.researcheridABH-8594-2020
dc.identifier.eissn15306992
dc.publisher.place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dcterms.isPartOf.abbreviationNano Lett.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-2143-2270-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPiJa938-
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