Characterization of the sigma(B) regulon in Staphylococcus aureus

DC FieldValueLanguage
dc.contributor.authorGertz, S
dc.contributor.authorEngelmann, S
dc.contributor.authorSchmid, R
dc.contributor.authorZiebandt, AK
dc.contributor.authorTischer, K
dc.contributor.authorScharf, C
dc.contributor.authorHacker, J
dc.contributor.authorHecker, M
dc.date.accessioned2021-12-23T16:13:42Z-
dc.date.available2021-12-23T16:13:42Z-
dc.date.issued2000
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10705-
dc.description.abstractThe sigma (B)-dependent stress regulon in gram-positive bacteria might fulfill a physiological role in stress response and virulence similar to that of the sigma (S) regulon in Escherichia coli and other gram-negative bacteria. in order to obtain evidence for the function of the sigma (B) regulon of Staphylococcus aureus, especially in virulence control, sigma (B)-dependent stress genes were identified. The two-dimensional protein pattern of wild-type cells of S. aureus COL was compared with that of an isogenic sigB mutant. By this approach, we found that the synthesis of about 27 cytoplasmic proteins seemed to be under the positive control of sigma (B). N-terminal sequencing of 18 proteins allowed the identification of their genes on the almost finished genome sequence of S, aureus COL and the analysis of the promoter structure. Transcriptional analyses of II of these genes confirmed their sigma (B) dependency, and moreover, about 7 additional sigma (B)-dependent genes were found which are cotranscribed with the newly detected genes, forming operons, Altogether, we identified 23 sigma (B)-dependent genes and their corresponding proteins, Among them are proteins probably involved in the generation of NADH or in membrane transport mechanisms, Furthermore, at Least one clpC-homologous gene was localized on the S, aureus sequence solely transcribed by sigma (B). In contrast, a second clpC-homologous gene in S, aureus forming an operon with ctsR, yacH, and yacI was sigma (B) independently expressed.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectBACILLUS-SUBTILIS
dc.subjectGENE
dc.subjectHEAT-SHOCK
dc.subjectLISTERIA-MONOCYTOGENES
dc.subjectMicrobiology
dc.subjectMYCOBACTERIUM-TUBERCULOSIS
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectSAR LOCUS
dc.subjectSTATIONARY-PHASE
dc.subjectSTRESS TRANSCRIPTION FACTOR-SIGMA(B)
dc.subjectSUBTILIS CLPC OPERON
dc.titleCharacterization of the sigma(B) regulon in Staphylococcus aureus
dc.typejournal article
dc.identifier.doi10.1128/JB.182.24.6983-6991.2000
dc.identifier.isiISI:000167293800016
dc.description.volume182
dc.description.issue24
dc.description.startpage6983
dc.description.endpage6991
dc.contributor.orcid0000-0001-6806-6450
dc.contributor.researcheridA-7931-2011
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
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