CHARACTERIZATION OF A LINEAR EXTRACHROMOSOMAL DNA ELEMENT (PBL1) ISOLATED AFTER INTERSPECIFIC MATING BETWEEN STREPTOMYCES-BAMBERGIENSIS AND STREPTOMYCES-LIVIDANS
| Autor(en): | ZOTCHEV, SB SOLDATOVA, LI OREKHOV, AV SCHREMPF, H |
Stichwörter: | DNA; ENDS; ESCHERICHIA-COLI; INITIATION; LINEAR; MATING; Microbiology; PHI-29; PHYSICAL MAP; PLASMID; PROTEINS; REPLICATION; STREPTOMYCES-BAMBERGIENSIS; STREPTOMYCES-LIVIDANS; TRANSFER | Erscheinungsdatum: | 1992 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | RESEARCH IN MICROBIOLOGY | Volumen: | 143 | Ausgabe: | 9 | Startseite: | 839 | Seitenende: | 845 | Zusammenfassung: | Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3'- and 5'-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains. |
ISSN: | 09232508 | DOI: | 10.1016/0923-2508(92)90071-U |
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