Regulation of plant cytosolic aldolase functions by redox-modifications

Autor(en): van der Linde, Karina
Gutsche, Nora 
Letters, Hans-Martin
Lindermayr, Christian
Mueller, Bernd
Holtgrefe, Simone
Scheibe, Renate 
Stichwörter: Arabidopsis thaliana; Biotin-switch assay; Cytosolic aldolase; DEHYDROGENASE; Disulfide bridge; GLUTAREDOXINS; Glutathionylation; GSNO; GSSG; IDENTIFICATION; METABOLIC ENZYMES; MUSCLE FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE; Nitrosylation; Nuclear localization; NUCLEAR-LOCALIZATION; Plant Sciences; PROTEIN S-GLUTATHIONYLATION; ROS; Split-YFP; SULFHYDRYL-GROUPS; THIOREDOXIN; Thioredoxin h; TRANSCRIPTION
Erscheinungsdatum: 2011
Volumen: 49
Ausgabe: 9
Startseite: 946
Seitenende: 957
From the five genes which code for cytosolic fructose 1,6-bisphosphate aldolases in Arabidopsis thaliana L, the cDNA clone of cAld2 (At2g36460), was heterologously expressed in E. coli and incubated under various oxidizing and reducing conditions. Covalent binding of a GSH moiety to the enzyme was shown by incorporation of biotinylated GSH (BioGEE) and by immunodetection with monoclonal anti-GSH serum. Nitrosylation after incubation with GSNO or SNP was demonstrated using the biotin-switch assay. Mass-spectrometry analysis showed glutathionylation and/or nitrosylation at two different cysteine residues: GSH was found to be attached to C68 and C173, while the nitroso-group was incorporated only into C173. Non-reducing SDS-PAGE conducted with purified wild-type and various Cysmutant proteins revealed the presence of disulfide bridges in the oxidized enzyme, as described for rabbit muscle aldolase. Incubation of the purified enzyme with GSSG (up to 25 mM) led to partial and reversible inactivation of enzyme activity; NADPH, in the presence of the components of the cytosolic NADP-dependent thioredoxin system, could reactivate the aldolase as did DTT. Total and irreversible inactivation occurred with low concentrations (0.1 mM) of nitrosoglutathione (GSNO). Inactivation was prevented by co-incubation of cAld2 with fructose-1,6-bisphosphate (FBP). Nuclear localization of cAld2 and interaction with thioredoxins was shown by transient expression of fusion constructs with fluorescent proteins in isolated protoplasts. (C) 2011 Elsevier Masson SAS. All rights reserved.
ISSN: 09819428
DOI: 10.1016/j.plaphy.2011.06.009

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