Correlation between growth rates, EIIA(Crr) phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12

Autor(en): Bettenbrock, Katja
Sauter, Thomas
Jahreis, Knut
Kremling, Andreas
Lengeler, Joseph W.
Gilles, Ernst-Dieter
Stichwörter: ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE; ADENYLATE-CYCLASE ACTIVITY; CATABOLITE REPRESSION; ENZYME-II; INDUCER EXCLUSION; MEDIATED REGULATION; METABOLIC PATHWAYS; Microbiology; PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE; SALMONELLA-TYPHIMURIUM; SIGNAL-TRANSDUCTION
Erscheinungsdatum: 2007
Herausgeber: AMER SOC MICROBIOLOGY
Journal: JOURNAL OF BACTERIOLOGY
Volumen: 189
Ausgabe: 19
Startseite: 6891
Seitenende: 6900
Zusammenfassung: 
In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIA(Crr) (EIIA(Crr)) (= EIIA(Glc)) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIA(Crr) (EIIA(Crr)similar to P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIA(Crr) to EIIA(Crr)similar to P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h(-1) and 0.7 h(-1), corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.
ISSN: 00219193
DOI: 10.1128/JB.00819-07

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