Correlation between growth rates, EIIA(Crr) phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12

DC FieldValueLanguage
dc.contributor.authorBettenbrock, Katja
dc.contributor.authorSauter, Thomas
dc.contributor.authorJahreis, Knut
dc.contributor.authorKremling, Andreas
dc.contributor.authorLengeler, Joseph W.
dc.contributor.authorGilles, Ernst-Dieter
dc.date.accessioned2021-12-23T16:13:59Z-
dc.date.available2021-12-23T16:13:59Z-
dc.date.issued2007
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10848-
dc.description.abstractIn Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIA(Crr) (EIIA(Crr)) (= EIIA(Glc)) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIA(Crr) (EIIA(Crr)similar to P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIA(Crr) to EIIA(Crr)similar to P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h(-1) and 0.7 h(-1), corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE
dc.subjectADENYLATE-CYCLASE ACTIVITY
dc.subjectCATABOLITE REPRESSION
dc.subjectENZYME-II
dc.subjectINDUCER EXCLUSION
dc.subjectMEDIATED REGULATION
dc.subjectMETABOLIC PATHWAYS
dc.subjectMicrobiology
dc.subjectPHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE
dc.subjectSALMONELLA-TYPHIMURIUM
dc.subjectSIGNAL-TRANSDUCTION
dc.titleCorrelation between growth rates, EIIA(Crr) phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12
dc.typejournal article
dc.identifier.doi10.1128/JB.00819-07
dc.identifier.isiISI:000249749700016
dc.description.volume189
dc.description.issue19
dc.description.startpage6891
dc.description.endpage6900
dc.contributor.orcid0000-0001-8225-2954
dc.contributor.researcheridAAN-5812-2020
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusBronze, Green Published
Show simple item record

Google ScholarTM

Check

Altmetric