COMPARATIVE-ANALYSIS OF SODIUM-DEPENDENT L-GLUTAMATE TRANSPORT OF SYNAPTOSOMAL AND ASTROGLIAL MEMBRANE-VESICLES FROM MOUSE CORTEX

Autor(en): RAUEN, T
JESERICH, G
DANBOLT, NC
KANNER, BI
Stichwörter: ACTIVE-TRANSPORT; ASTROGLIAL MEMBRANE VESICLE; BINDING; Biochemistry & Molecular Biology; Biophysics; Cell Biology; GAMMA-AMINOBUTYRIC-ACID; GLIAL CELLS; GLUTAMATE ANALOG; HIGH-AFFINITY UPTAKE; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; L-ASPARTATE; L-GLUTAMATE TRANSPORT; METABOLISM; PROTEINS; RAT-BRAIN SLICES; SYNAPTOSOME; UPTAKE SYSTEMS
Erscheinungsdatum: 1992
Herausgeber: ELSEVIER SCIENCE BV
Enthalten in: FEBS LETTERS
Band: 312
Ausgabe: 1
Startseite: 15
Seitenende: 20
Zusammenfassung: 
Uptake of [H-3]L-glutamate into membrane vesicles prepared from either mouse cortical astrocyte cultures or synaptosomes was found to be an electrogenic sodium- and potassium-dependent transport process with saturable uptake kinetics. Pharmacological differences were revealed by using a variety of substrate analogues. L-trans-PDC inhibited the synaptosomal glutamate transport 2-4-fold stronger than the astroglial uptake. The substrate analogues DL-threo-beta-hydroxy-aspartate, DL-aspartate-beta-hydroxamate, L-aspartate and D-aspartate inhibited glutamate transport of astroglial and neuronal membrane vesicles in a distinctive manner, whereas D-glutamate, quisqualate and dihydrokainate had no effect in either case. Immunoblotting and immunocytochemical labeling with antibodies against the rat brain glutamate transporter revealed the selective reaction of a band at about 75 kDa mol. wt. and a specific pattern of astrocyte immunostaining.
ISSN: 00145793
DOI: 10.1016/0014-5793(92)81401-7

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