Retromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles

DC FieldValueLanguage
dc.contributor.authorArlt, Henning
dc.contributor.authorReggiori, Fulvio
dc.contributor.authorUngermann, Christian
dc.date.accessioned2021-12-23T16:14:01Z-
dc.date.available2021-12-23T16:14:01Z-
dc.date.issued2015
dc.identifier.issn00219533
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10866-
dc.description.abstractEndosomes are dynamic organelles that need to combine the ability to successfully deliver proteins and lipids to the lysosome-like vacuole, and recycle others to the Golgi or the plasma membrane. We now show that retromer, which is implicated in retrieval of proteins from endosomes to the Golgi or to the plasma membrane, can act on vacuoles. We explore its function using an assay that allows us to dissect the required cofactors during recycling. We demonstrate that recycling of the transmembrane receptor Vps10 from vacuoles requires the retromer, the dynamin-like Vps1, and the Rab7 GTPase Ypt7. Retromer and Vps1 leave the vacuole together with the cargo, whereas Ypt7 stays behind, in agreement with its regulatory function. Recycled cargo then accumulates at endosomes and later at the Golgi, implying consecutive sorting steps to the final destination. Our data further suggest that retromer and Vps1 are essential to maintain vacuole membrane organization. Taken together, our data demonstrate that retromer can cooperate with Vps1 and the Rab Ypt7 to clear the vacuole of selected membrane proteins.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG); Hans-Muhlenhoff foundation; Deutsche Akademische Auslandsdienst (DAAD)Deutscher Akademischer Austausch Dienst (DAAD); NWO (Netherlands Organisation for Scientific Research)Netherlands Organization for Scientific Research (NWO) [821.02.017, 822.02.014]; DFG-NWOGerman Research Foundation (DFG)Netherlands Organization for Scientific Research (NWO) [DN82-303]; Netherlands Organisation for Health Research and Development (ZonMW) VICI [016.130.606]; [Sonderforschungbereich (SFB) 944]; This work was supported by the Deutsche Forschungsgemeinschaft (DFG); Sonderforschungbereich (SFB) 944 (project P11); and by the Hans-Muhlenhoff foundation (to C.U.). H.A. received support by a fellowship of the Deutsche Akademische Auslandsdienst (DAAD). F.R. is supported by ALW Open Program of the NWO (Netherlands Organisation for Scientific Research) [grant numbers 821.02.017 and 822.02.014]; a DFG-NWO cooperation [grant number DN82-303]; and The Netherlands Organisation for Health Research and Development (ZonMW) VICI [grant number 016.130.606].
dc.language.isoen
dc.publisherCOMPANY BIOLOGISTS LTD
dc.relation.ispartofJOURNAL OF CELL SCIENCE
dc.subjectCARGO-RECOGNITION
dc.subjectCell Biology
dc.subjectCLATHRIN
dc.subjectCOMPARTMENTS
dc.subjectCOMPLEX
dc.subjectDynamin
dc.subjectEndosomal trafficking
dc.subjectENDOSOME
dc.subjectGOLGI RETROGRADE TRANSPORT
dc.subjectRECRUITMENT
dc.subjectRecycling
dc.subjectREQUIRES
dc.subjectRetromer
dc.subjectSORTING RECEPTOR
dc.subjectVacuole
dc.subjectVERSATILE
dc.subjectVps1
dc.titleRetromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles
dc.typejournal article
dc.identifier.doi10.1242/jcs.132720
dc.identifier.isiISI:000349786500005
dc.description.volume128
dc.description.issue4
dc.description.startpage645
dc.description.endpage655
dc.contributor.orcid0000-0001-6395-9620
dc.contributor.orcid0000-0003-2652-2686
dc.contributor.researcheridU-8327-2019
dc.identifier.eissn14779137
dc.publisher.placeBIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND
dcterms.isPartOf.abbreviationJ. Cell Sci.
dcterms.oaStatusGreen Submitted, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6395-9620-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidArHe830-
crisitem.author.netidUnCh999-
Show simple item record

Page view(s)

1
Last Week
0
Last month
0
checked on May 27, 2024

Google ScholarTM

Check

Altmetric