Lack of the NAD(+)-dependent glycerol 3-phosphate dehydrogenase impairs the function of transcription factors Sip4 and Cat8 required for ethanol utilization in Kluyveromyces lactis
DC Element | Wert | Sprache |
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dc.contributor.author | Mojardin, Lucia | |
dc.contributor.author | Vega, Montserrat | |
dc.contributor.author | Moreno, Fernando | |
dc.contributor.author | Schmitz, Hans-Peter | |
dc.contributor.author | Heinisch, Juergen J. | |
dc.contributor.author | Rodicio, Rosaura | |
dc.date.accessioned | 2021-12-23T16:14:04Z | - |
dc.date.available | 2021-12-23T16:14:04Z | - |
dc.date.issued | 2018 | |
dc.identifier.issn | 10871845 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10889 | - |
dc.description.abstract | The NAD(+)-dependent glycerol 3-phosphate dehydrogenase (KlGpd1) is an important enzyme for maintenance of the cytosolic redox balance in the milk yeast Kluyveromyces lactis. The enzyme is localized in peroxisomes and in the cytosol, indicating its requirement for the oxidation of NADH in both compartments. Klgpd1 mutants grow more slowly on glucose than wild-type cells and do not grow on ethanol as a sole carbon source. We studied the molecular basis of the latter phenotype and found that Gpd1 is required for high expression of KlICL1 and KlMLS1 which encode the key enzymes of the glyoxylate pathway isocitrate lyase and malate synthase, respectively. This regulation is mediated by CSRE elements in the promoters of these genes and the Snf1-regulated transcription factors KlCat8 and KlSip4. To study the transactivation function of these factors we developed a modified yeast one-hybrid system for K. lactis, using the endogenous beta-galactosidase gene LAC4 as a reporter in a lac9 deletion background. In combination with ChIP analyses we discovered that Gpd1 controls both the specific binding of Cat8 and Sip4 to the target promoters and the capacity of these factors to activate the reporter gene expression. We propose a model in which KlGpd1 activity is required for maintenance of the redox balance. In its absence, genes which function in generating redox balance instabilities are not expressed. A comparison of mutant phenotypes further indicates, that this system not only operates in K. lactis, but also in Saccharomyces cerevisiae. | |
dc.description.sponsorship | University of Oviedo (Spain); FPI fellowship by the MICINN (Spain); MICINN (Spain)Spanish Government [BFU2010-19628-C02-01]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [HE 1880/6-1]; We thank Andrea Murra for excellent technical assistance. L.M. was supported by a fellowship provided by the University of Oviedo (Spain) and M.V. by a FPI fellowship provided by the MICINN (Spain). This work was funded by a grant BFU2010-19628-C02-01 from the MICINN (Spain). The laboratory of JJH received a grant from the Deutsche Forschungsgemeinschaft (HE 1880/6-1). | |
dc.language.iso | en | |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | |
dc.relation.ispartof | FUNGAL GENETICS AND BIOLOGY | |
dc.subject | ACTIVATION | |
dc.subject | BUDDING YEAST | |
dc.subject | Carbon stress response | |
dc.subject | CATABOLITE REPRESSION | |
dc.subject | Genetics & Heredity | |
dc.subject | GLUCONEOGENIC GENES | |
dc.subject | Glyoxylate cycle | |
dc.subject | ISOCITRATE LYASE | |
dc.subject | METABOLISM | |
dc.subject | Mycology | |
dc.subject | Peroxisome | |
dc.subject | PROTEIN-KINASE | |
dc.subject | Redox balance | |
dc.subject | SNF1 | |
dc.subject | STRESS | |
dc.subject | YEAST SACCHAROMYCES-CEREVISIAE | |
dc.title | Lack of the NAD(+)-dependent glycerol 3-phosphate dehydrogenase impairs the function of transcription factors Sip4 and Cat8 required for ethanol utilization in Kluyveromyces lactis | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.fgb.2017.11.006 | |
dc.identifier.isi | ISI:000425484800003 | |
dc.description.volume | 111 | |
dc.description.startpage | 16 | |
dc.description.endpage | 29 | |
dc.contributor.orcid | 0000-0001-5449-4593 | |
dc.contributor.orcid | 0000-0002-4837-0671 | |
dc.contributor.researcherid | G-3801-2017 | |
dc.contributor.researcherid | ABE-7870-2021 | |
dc.identifier.eissn | 10960937 | |
dc.publisher.place | 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA | |
dcterms.isPartOf.abbreviation | Fungal Genet. Biol. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0001-5449-4593 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | ScHa130 | - |
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geprüft am 01.06.2024