The structure of the V-1-ATPase determined by three-dimensional electron microscopy of single particles

DC FieldValueLanguage
dc.contributor.authorRadermacher, M
dc.contributor.authorRuiz, T
dc.contributor.authorWieczorek, H
dc.contributor.authorGruber, G
dc.date.accessioned2021-12-23T16:14:33Z-
dc.date.available2021-12-23T16:14:33Z-
dc.date.issued2001
dc.identifier.issn10478477
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11126-
dc.description.abstractWe determined the structure of the V-1-ATPase from Manduca sexta to a resolution of 1.8 nm, which for the first time reveals internal features of the enzyme. The V-1-ATPase consists of a headpiece of 13.5 nm in diameter, with six elongated subunits, A(3) and B-3, of approximately equal size, and a stalk of 6 nm in length that connects V-1 with the membrane-bound domain, V-O. At the center of the molecule is a cavity that extends throughout the length of the A(3)B(3) hexamer. Inside the cavity the central stalk can be seen connected to only two of the catalytic A subunits. The structure was obtained by a combination of the Random Conical Reconstruction Technique and angular refinements. Additional recently developed techniques that were used include methods for simultaneous translational rotational alignment of the 0 degrees images, contrast transfer function correction for tilt images, and the Two-Step Radon Inversion Algorithm. (C) 2001 Academic Press.
dc.description.sponsorshipNCRR NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [P41 RR01219] Funding Source: Medline; NIAMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [5RO1 AR40615] Funding Source: Medline; NIGMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01 GM29169] Funding Source: Medline; NATIONAL CENTER FOR RESEARCH RESOURCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [P41RR001219] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [R01AR040615] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM029169] Funding Source: NIH RePORTER
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofJOURNAL OF STRUCTURAL BIOLOGY
dc.subject3-DIMENSIONAL RECONSTRUCTION
dc.subjectALIGNMENT
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectCTF correction
dc.subjectelectron microscopy
dc.subjectESCHERICHIA-COLI
dc.subjectF-1 ATPASE
dc.subjectF-1-ATPASE
dc.subjectH+V-ATPase
dc.subjectManduca sexta
dc.subjectPROJECTIONS
dc.subjectRADON TRANSFORMS
dc.subjectRESOLUTION
dc.subjectsimultaneous alignment
dc.subjectSUBUNIT
dc.subjectthree-dimensional reconstruction
dc.subjectTILT SERIES
dc.subjectvacuolar-type ATPase
dc.titleThe structure of the V-1-ATPase determined by three-dimensional electron microscopy of single particles
dc.typejournal article
dc.identifier.doi10.1006/jsbi.2001.4395
dc.identifier.isiISI:000171447400004
dc.description.volume135
dc.description.issue1
dc.description.startpage26
dc.description.endpage37
dc.identifier.eissn10958657
dc.publisher.place525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
dcterms.isPartOf.abbreviationJ. Struct. Biol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWiHe990-
Show simple item record

Page view(s)

3
Last Week
0
Last month
0
checked on May 27, 2024

Google ScholarTM

Check

Altmetric