DC Field | Value | Language |
dc.contributor.author | Rieger, Bettina | |
dc.contributor.author | Shalaeva, Daria N. | |
dc.contributor.author | Soehnel, Anna-Carina | |
dc.contributor.author | Kohl, Wladislaw | |
dc.contributor.author | Duwe, Patrick | |
dc.contributor.author | Mulkidjanian, Armen Y. | |
dc.contributor.author | Busch, Karin B. | |
dc.date.accessioned | 2021-12-23T16:14:49Z | - |
dc.date.available | 2021-12-23T16:14:49Z | - |
dc.date.issued | 2017 | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/11260 | - |
dc.description.abstract | The assembly of respiratory complexes into macromolecular supercomplexes is currently a hot topic, especially in the context of newly available structural details. However, most work to date has been done with purified detergent-solubilized material and in situ confirmation is absent. We here set out to enable the recording of respiratory supercomplex formation in living cells. Fluorescent sensor proteins were placed at specific positions at cytochrome c oxidase suspected to either be at the surface of a CI1CIII2CIV1 supercomplex or buried within this supercomplex. In contrast to other loci, sensors at subunits CoxVIIIa and CoxVIIc reported a dense protein environment, as detected by significantly shortened fluorescence lifetimes. According to 3D modelling CoxVIIIa and CoxVIIc are buried in the CI1CIII2CIV1 supercomplex. Suppression of supercomplex scaffold proteins HIGD2A and CoxVIIa2i was accompanied by an increase in the lifetime of the CoxVIIIa-sensor in line with release of CIV from supercomplexes. Strikingly, our data provide strong evidence for defined stable supercomplex configuration in situ. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [Bu2288/1-2]; Osnabrueck University; German Academic Exchange ServiceDeutscher Akademischer Austausch Dienst (DAAD); Russian Science Foundation (DNS)Russian Science Foundation (RSF) [14-50-00029]; [CRC-944]; This work supported by the CRC-944 and DFG grant Bu2288/1-2 (KB), the Osnabrueck University, the German Academic Exchange Service, and the grant 14-50-00029 from the Russian Science Foundation (DNS). We thank Iven Winckelmann for important preliminary studies and Guy Hanke for critically reading and improving the manuscript. | |
dc.language.iso | en | |
dc.publisher | NATURE RESEARCH | |
dc.relation.ispartof | SCIENTIFIC REPORTS | |
dc.subject | ARCHITECTURE | |
dc.subject | COMPLEX-I | |
dc.subject | CYTOCHROME-C-OXIDASE | |
dc.subject | ELECTRON | |
dc.subject | LIVING CELLS | |
dc.subject | LOCALIZATION | |
dc.subject | MITOCHONDRIAL MICROCOMPARTMENTS | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | RELEVANCE | |
dc.subject | RESPIRASOME | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | YEAST | |
dc.title | Lifetime imaging of GFP at CoxVIIIa reports respiratory supercomplex assembly in live cells | |
dc.type | journal article | |
dc.identifier.doi | 10.1038/srep46055 | |
dc.identifier.isi | ISI:000398549000001 | |
dc.description.volume | 7 | |
dc.contributor.orcid | 0000-0001-5844-3064 | |
dc.contributor.orcid | 0000-0003-0525-0191 | |
dc.contributor.orcid | 0000-0003-0582-2612 | |
dc.contributor.researcherid | J-8086-2013 | |
dc.contributor.researcherid | ABH-8594-2020 | |
dc.contributor.researcherid | AAH-3608-2021 | |
dc.contributor.researcherid | AAM-8374-2021 | |
dc.publisher.place | HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY | |
dcterms.isPartOf.abbreviation | Sci Rep | |
dcterms.oaStatus | Green Published, gold | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | RiBe842 | - |
crisitem.author.netid | SoAn320 | - |
crisitem.author.netid | KoWl001 | - |
crisitem.author.netid | DuPa001 | - |