FRET (fluorescence resonance energy transfer) sheds light on transcription
| Autor(en): | Grohmann, Dina Klose, Daniel Fielden, Daniel Werner, Finn |
Stichwörter: | BINDING; Biochemistry & Molecular Biology; COMPLEX; fluorescence resonance energy transfer (FRET); IN-VITRO; INITIATION; MOLECULAR-MECHANISMS; NANO-POSITIONING SYSTEM; PROMOTER DNA; RNA polymerase; RNA-POLYMERASE-II; SPECTROSCOPY; SUBUNITS F/E; TATA-box-binding protein (TBP); transcription | Erscheinungsdatum: | 2011 | Herausgeber: | PORTLAND PRESS LTD | Journal: | BIOCHEMICAL SOCIETY TRANSACTIONS | Volumen: | 39 | Ausgabe: | 1 | Startseite: | 122 | Seitenende: | 127 | Zusammenfassung: | The complex organization of the transcription machinery has been revealed mainly by biochemical and crystallographic studies. X-ray structures describe RNA polymerases and transcription complexes on an atomic level, but fail to portray their dynamic nature. The use of fluorescence techniques has made it possible to add a new layer of information to our understanding of transcription by providing details about the structural rearrangement of mobile elements and the network of interactions within transcription complexes in solution and in real-time. |
ISSN: | 03005127 | DOI: | 10.1042/BST0390122 |
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geprüft am 16.05.2024
