The chloroplast protein import channel Toc75: Pore properties and interaction with transit peptides

DC FieldValueLanguage
dc.contributor.authorHinnah, SC
dc.contributor.authorWagner, R
dc.contributor.authorSveshnikova, N
dc.contributor.authorHarrer, R
dc.contributor.authorSoll, J
dc.date.accessioned2021-12-23T16:15:11Z-
dc.date.available2021-12-23T16:15:11Z-
dc.date.issued2002
dc.identifier.issn00063495
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11324-
dc.description.abstractThe channel properties of Toc75 (the protein import pore of the outer chloroplastic membrane) were further characterized by electrophysiological measurements in planar lipid bilayers. After improvement of the Toc75 reconstitution procedure the voltage dependence of the channel open probability resembled those observed for other beta-barrel pores. Studies concerning the pore size of the reconstituted Toc75 indicate the presence of a narrow restriction zone corresponding to the selectivity filter and a wider pore vestibule with diameters of similar to14 Angstrom , and 26 Angstrom, respectively. Interactions between Toc75 and different peptides (a genuine chloroplastic transit peptide, a synthetic peptide resembling a transit peptide, and a mitochondrial presequence) show that Toc75 itself is able to differentiate between these peptides and the recognition is based on both conformational and electrostatic interactions.
dc.language.isoen
dc.publisherBIOPHYSICAL SOCIETY
dc.relation.ispartofBIOPHYSICAL JOURNAL
dc.subjectACID CHAIN
dc.subjectBILAYER-MEMBRANES
dc.subjectBiophysics
dc.subjectENVELOPE MEMBRANES
dc.subjectESCHERICHIA-COLI
dc.subjectION CHANNELS
dc.subjectMITOCHONDRIAL
dc.subjectOUTER-MEMBRANE
dc.subjectPHOSPHOLIPID-VESICLES
dc.subjectPLANAR MEMBRANE
dc.subjectPRECURSOR PROTEINS
dc.titleThe chloroplast protein import channel Toc75: Pore properties and interaction with transit peptides
dc.typejournal article
dc.identifier.doi10.1016/S0006-3495(02)75216-8
dc.identifier.isiISI:000177197900023
dc.description.volume83
dc.description.issue2
dc.description.startpage899
dc.description.endpage911
dc.contributor.orcid0000-0002-9194-516X
dc.publisher.place9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA
dcterms.isPartOf.abbreviationBiophys. J.
dcterms.oaStatusBronze, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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