Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: Characterization of the gbsAB genes

DC FieldValueLanguage
dc.contributor.authorBoch, J
dc.contributor.authorKempf, B
dc.contributor.authorSchmid, R
dc.contributor.authorBremer, E
dc.date.accessioned2021-12-23T16:15:12Z-
dc.date.available2021-12-23T16:15:12Z-
dc.date.issued1996
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11328-
dc.description.abstractSynthesis of the osmoprotectant glycine betaine from the exogenously provided precursor choline or glycine betaine aldehyde confers considerable osmotic stress tolerance to Bacillus subtilis in high-osmolarity media. Using an Escherichia coli mutant (betBA) defective in the glycine betaine synthesis enzymes, we cloned by functional complementation the genes that are required for the synthesis of the osmoprotectant glycine betaine in B. subtilis. The DNA sequence of a 4.1-kb segment from the cloned chromosomal B. subtilis DNA was established, and two genes (gbsA and gbsB) whose products were essential for glycine betaine biosynthesis and osmoprotection were identified. The gbsA and gbsB genes are transcribed in the same direction, are separated by a short intergenic region, and are likely to form an operon. The deduced gbsA gene product exhibits strong sequence identity with members of a superfamily of specialized and nonspecialized aldehyde dehydrogenases. This superfamily comprises glycine betaine aldehyde dehydrogenases from bacteria and plants with known involvement in the cellular adaptation to high-osmolarity stress and drought. The deduced gbsB gene product shows significant similarity to the family of type III alcohol dehydrogenases. B. subtilis mutants with defects in the chromosomal gbsAB genes were constructed by marker replacement, and the growth properties of these mutant strains in high-osmolarity medium were analyzed. Deletion of the gbsAB genes destroyed the choline-glycine betaine synthesis pathway and abolished the ability of B. subtilis to deal effectively with high-osmolarity stress in choline- or glycine betaine aldehyde-containing medium. Uptake of radiolabelled choline was unaltered in the gbsAB mutant strain. The continued intracellular accumulation of choline or glycine betaine aldehyde in a strain lacking the glycine betaine-biosynthetic enzymes strongly interfered with the growth of B. subtilis, even in medium of moderate osmolarity. A single transcription initiation site for gbsAB was detected by high-resolution primer extension analysis. gbsAB transcription was initiated from a promoter with close homology to sigma(A)-dependent promoters and was stimulated by the presence of choline in the growth medium.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectALDEHYDE DEHYDROGENASE
dc.subjectCHOLINE-OXIDASE
dc.subjectCOPY-NUMBER
dc.subjectDEPENDENT TRANSPORT-SYSTEM
dc.subjectESCHERICHIA-COLI
dc.subjectHIGH-LEVEL
dc.subjectHIGHER-PLANTS
dc.subjectMicrobiology
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectOSMOTIC-STRESS
dc.subjectRNA-POLYMERASE
dc.titleSynthesis of the osmoprotectant glycine betaine in Bacillus subtilis: Characterization of the gbsAB genes
dc.typejournal article
dc.identifier.doi10.1128/jb.178.17.5121-5129.1996
dc.identifier.isiISI:A1996VE37100010
dc.description.volume178
dc.description.issue17
dc.description.startpage5121
dc.description.endpage5129
dc.publisher.place1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
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