Regulation of sigma(B)-dependent transcription of sigB and asp23 in two different Staphylococcus aureus strains

DC FieldValueLanguage
dc.contributor.authorGertz, S
dc.contributor.authorEngelmann, S
dc.contributor.authorSchmid, R
dc.contributor.authorOhlsen, K
dc.contributor.authorHacker, J
dc.contributor.authorHecker, M
dc.date.accessioned2021-12-23T16:15:44Z-
dc.date.available2021-12-23T16:15:44Z-
dc.date.issued1999
dc.identifier.issn00268925
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11559-
dc.description.abstractThe alkaline shock protein Asp23 was identified as a sigma(B)-dependent protein in Staphylococcus aureus. In Bacillus subtilis, the asp23 promoter from S. aureus is regulated like other sigma(B)-depondent promoters, which are strongly induced by heat and ethanol stress. However, almost no induction of asp23 expression was found after heat or ethanol stress in S, aureus MA13 grown in a synthetic medium, where the basal expression level of asp23 is high. Under the same experimental conditions the sigma(B) gene itself showed a similar expression pattern: it was highly expressed in synthetic medium but not induced by heat or ethanol stress. In contrast, sigma(B) activity was increased by heat stress when the cells were grown in a complex medium. The constitutive expression of sigB and sigma(B)-dependent stress genes in S. aureus MA13 grown in a synthetic medium is in a sharp contrast to the regulation of sigma(B) activity in B. subtilis, and needs further investigation. A deletion of 11 bp in the rsbU gene, which encodes the phosphatase that acts on RsbV (the anti-anti-sigma factor), in S. aureus NCTC 8325-4 might be responsible for the failure of heat stress to activate sigma(B) in complex medium, and thus reduce the initiation of transcription at sigma(B)-dependent promoters in this strain.
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofMOLECULAR AND GENERAL GENETICS
dc.subjectasp23
dc.subjectBacillus subtilis
dc.subjectBACILLUS-SUBTILIS
dc.subjectBACTERIAL
dc.subjectBiochemistry & Molecular Biology
dc.subjectENVIRONMENTAL-STRESS
dc.subjectFACTOR SIGMA(B)
dc.subjectGenetics & Heredity
dc.subjectHEAT-SHOCK
dc.subjectLISTERIA-MONOCYTOGENES
dc.subjectPROTEIN
dc.subjectRESISTANCE
dc.subjectrsbU
dc.subjectsigB
dc.subjectStaphylococcus aureus
dc.subjectSTARVATION-SURVIVAL
dc.subjectVIRULENCE
dc.titleRegulation of sigma(B)-dependent transcription of sigB and asp23 in two different Staphylococcus aureus strains
dc.typejournal article
dc.identifier.doi10.1007/s004380051001
dc.identifier.isiISI:000079888700017
dc.description.volume261
dc.description.issue3
dc.description.startpage558
dc.description.endpage566
dc.publisher.place233 SPRING ST, NEW YORK, NY 10013 USA
dcterms.isPartOf.abbreviationMol. Gen. Genet.
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