1-DEAZA AND 3-DEAZAPURINES IN THE REACTION OF MICROBIOLOGICAL TRANSGLYCOSYLATION

Abstract

Substrate activity of 1- and 3-deazapurines in the reaction of microbiological ribo- 2-deoxyribosylation catalysed by purine nucleoside phosphorylase of viable cells of E. coli BMT-1D/1A has been studied. Guanosine or 2'-deoxyguanosine were used as donors. 1-Deazapurines are good substrates in both reactions; 3-deazapurines are very effective in transdeoxyribosylation but not in transribosylation. Benzimidazole is an excellent substrate in both reactions indicating that N(1) and N(3) are not essential for transglycosylation.