DC Element | Wert | Sprache |
dc.contributor.author | SAHINTOTH, M | |
dc.contributor.author | FRILLINGOS, S | |
dc.contributor.author | LENGELER, JW | |
dc.contributor.author | KABACK, HR | |
dc.date.accessioned | 2021-12-23T16:15:50Z | - |
dc.date.available | 2021-12-23T16:15:50Z | - |
dc.date.issued | 1995 | |
dc.identifier.issn | 0006291X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/11606 | - |
dc.description.abstract | The cscB gene encoding a putative sucrose permease from Escherichia coli EC3132 [Bockmann, J., Heuel, H. & Lengeler, J. W. (1992) Mol. Gen. Genet. 235, 22-32] was placed under control of the lacZ promoter/operator and overexpressed. Cells of E. coli K-12 expressing cscB gene product from a high copy number plasmid accumulate sucrose against a concentration gradient with an apparent K-m of about 1 mM and a V-max of approximately 23 nmol/min/mg protein. In contrast, transport of lactose or melibiose is not observed. Epitope-tagging and immunological analysis reveal that concomitant with increased transport activity, a protein with an apparent M(r) of 33-34 kDa appears in the cytoplasmic membrane. The results provide direct support for the contention that the cscB gene encodes a sucrose permease and provide the basis for further molecular analysis. (C) 1995 Academic Press, Inc. | |
dc.language.iso | en | |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | |
dc.relation.ispartof | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biophysics | |
dc.subject | LAC CARRIER PROTEIN | |
dc.subject | LACTOSE PERMEASE | |
dc.subject | MEMBRANE | |
dc.subject | PURIFICATION | |
dc.title | ACTIVE-TRANSPORT BY THE CSCB PERMEASE IN ESCHERICHIA-COLI K-12 | |
dc.type | journal article | |
dc.identifier.doi | 10.1006/bbrc.1995.1449 | |
dc.identifier.isi | ISI:A1995QP67700031 | |
dc.description.volume | 208 | |
dc.description.issue | 3 | |
dc.description.startpage | 1116 | |
dc.description.endpage | 1123 | |
dc.contributor.researcherid | AAQ-3148-2021 | |
dc.identifier.eissn | 10902104 | |
dc.publisher.place | 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA | |
dcterms.isPartOf.abbreviation | Biochem. Biophys. Res. Commun. | |