Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors

DC FieldValueLanguage
dc.contributor.authorNaumann, Hendrik
dc.contributor.authorKlare, Johann P.
dc.contributor.authorEngelhard, Martin
dc.contributor.authorHildebrandt, Peter
dc.contributor.authorMurgida, Daniel H.
dc.date.accessioned2021-12-23T16:15:54Z-
dc.date.available2021-12-23T16:15:54Z-
dc.date.issued2006
dc.identifier.issn1474905X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11639-
dc.description.abstractThis article describes a method, based on surface-enhanced resonance Raman (SERR) spectroscopy, for studying the reaction dynamics of photoreceptors immobilized on metal electrodes. Time resolution and fresh sample conditions are achieved by synchronizing the rotational and translational motion of a novel kinematic electrode with the duration and time delay between the pump and probe events. The power and sensitivity of the method is illustrated by studying the photocycle of the sensory photoreceptor NpSRII and its sensitivity to the applied electric field. The results are compared with time-resolved resonance Raman measurements in solution.
dc.language.isoen
dc.publisherSPRINGERNATURE
dc.relation.ispartofPHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
dc.subjectABSORPTION
dc.subjectACTIVE-SITE STRUCTURE
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectChemistry
dc.subjectChemistry, Physical
dc.subjectCYTOCHROME-C
dc.subjectELECTRON-TRANSFER
dc.subjectOXIDASE
dc.subjectPHARAONIS
dc.subjectPHOTOCYCLE
dc.subjectREDOX PROCESSES
dc.subjectSENSORY RHODOPSIN-II
dc.subjectSPECTROSCOPY
dc.titleTime-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors
dc.typereview
dc.identifier.doi10.1039/b607807a
dc.identifier.isiISI:000243193700006
dc.description.volume5
dc.description.issue12
dc.description.startpage1103
dc.description.endpage1108
dc.contributor.orcid0000-0003-1030-5900
dc.contributor.orcid0000-0002-5761-5968
dc.contributor.researcheridJ-5658-2014
dc.contributor.researcheridC-1428-2009
dc.identifier.eissn14749092
dc.publisher.placeCAMPUS, 4 CRINAN ST, LONDON, N1 9XW, ENGLAND
dcterms.isPartOf.abbreviationPhotochem. Photobiol. Sci.
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