Debris buster is a Drosophila scavenger receptor essential for airway physiology

DC FieldValueLanguage
dc.contributor.authorWingen, Almut
dc.contributor.authorCarrera, Pilar
dc.contributor.authorPsathaki, Olympia Ekaterini
dc.contributor.authorVoelzmann, Andre
dc.contributor.authorPaululat, Achim
dc.contributor.authorHoch, Michael
dc.date.accessioned2021-12-23T16:16:04Z-
dc.date.available2021-12-23T16:16:04Z-
dc.date.issued2017
dc.identifier.issn00121606
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11709-
dc.description.abstractScavenger receptors class B (SR-B) are multifunctional transmembrane proteins, which in vertebrates participate in lipid transport, pathogen clearance, lysosomal delivery and intracellular sorting. Drosophila has 14 SR-B members whose functions are still largely unknown. Here, we reveal a novel role for the SR-B family member Debris buster (Dsb) in Drosophila airway physiology. Larvae lacking dsb show yeast avoidance behavior, hypoxia, and severe growth defects associated with impaired elongation and integrity along the airways. Furthermore, in dsb mutant embryos, the barrier function of the posterior spiracles, which are critical for gas exchange, is not properly established and liquid clearance is locally impaired at the spiracular lumen. We found that Dsb is specifically expressed in a group of distal epithelial cells of the posterior spiracle organ and not throughout the entire airways. Furthermore, tissue-specific knockdown and rescue experiments demonstrate that Dsb function in the airways is only required in the posterior spiracles. Dsb localizes in intracellular vesicles, and a subset of these associate with lysosomes. However, we found that depletion of proteins involved in vesicular transport to the apical membrane, but not in lysosomal function, causes dsb-like airway elongation defects. We propose a model in which Dsb sorts components of the apical extracellular matrix which are essential for airway physiology. Since SR-B LIMP2-deficient mice show reduced expression of several apical plasma membrane proteins, sorting of proteins to the apical membrane is likely an evolutionary conserved function of Dsb and LIMP2. Our data provide insights into a spatially confined function of the SR-B Dsb in intracellular trafficking critical for the physiology of the whole tubular airway network.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [SFB 645, TRR 83]; Excellence Cluster ImmunoSensation; Helmholtz Cross Program topic ``Metabolic Dysfunction''; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944, PA 517/12-1]; State of Lower-Saxony [ZN2832]; OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [P40OD010949] Funding Source: NIH RePORTER; This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) to M.H. and P.C. (SFB 645, TRR 83, and the Excellence Cluster ImmunoSensation) and the Helmholtz Cross Program topic ``Metabolic Dysfunction''. A.P and O.E.P. were supported by grants from the Deutsche Forschungsgemeinschaft (SFB 944 and PA 517/12-1) and from the State of Lower-Saxony (ZN2832).
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofDEVELOPMENTAL BIOLOGY
dc.subjectAirways
dc.subjectApical extracellular matrix
dc.subjectB TYPE-I
dc.subjectBARRIER FORMATION
dc.subjectBarrier function
dc.subjectCELLS
dc.subjectCUTICLE DIFFERENTIATION
dc.subjectDevelopmental Biology
dc.subjectDrosophila
dc.subjectMELANOGASTER
dc.subjectPHEROMONE DETECTION
dc.subjectScavenger receptors
dc.subjectSIGNAL-TRANSDUCTION
dc.subjectSpiracles
dc.subjectSR-BI
dc.subjectTRACHEAL SYSTEM
dc.subjectTUBE SIZE
dc.titleDebris buster is a Drosophila scavenger receptor essential for airway physiology
dc.typejournal article
dc.identifier.doi10.1016/j.ydbio.2017.08.018
dc.identifier.isiISI:000411302100006
dc.description.volume430
dc.description.issue1
dc.description.startpage52
dc.description.endpage68
dc.contributor.orcid0000-0002-7682-5637
dc.contributor.orcid0000-0002-8845-6859
dc.identifier.eissn1095564X
dc.publisher.place525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
dcterms.isPartOf.abbreviationDev. Biol.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPaAc947-
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