Distribution and evolution of chitinase genes in Streptomyces species: involvement of gene-duplication and domain-deletion

DC FieldValueLanguage
dc.contributor.authorSaito, A
dc.contributor.authorFujii, T
dc.contributor.authorMiyashita, K
dc.date.accessioned2021-12-23T16:16:21Z-
dc.date.available2021-12-23T16:16:21Z-
dc.date.issued2003
dc.identifier.issn00036072
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11828-
dc.description.abstractStreptomyces coelicolor A3(2) possesses nine genes for family 18 chitinases and two for family 19, showing high multiplicity. By hybridization analyses, distribution of those chitinase genes was investigated in six other Streptomyces species covering the whole phylogenetic range based on 16S rDNA sequences. All strains showed high-multiplicity of chitinase genes, like S. coelicolor A3(2). The phylogeny and gene organization of the family 18 chitinase genes cloned from Streptomyces species so far were then analyzed to investigate the gene evolution. It was concluded that Streptomyces already possessed a variety of chitinase genes prior to branching into many species, and that the ancestral genes of chiA and chiB have been generated by gene-duplication. In the course of the analyses, evidence that the chi30 and chi40 genes of S. thermoviolaceus were derived from their corresponding original chitinase genes by losing gene parts for substrate-binding domains and fibronectin type III-like domains was obtained. It was thus shown that gene-duplication and domain-deletion were implicated in generating the high diversity and multiplicity of chitinase genes in Streptomyces species.
dc.language.isoen
dc.publisherKLUWER ACADEMIC PUBL
dc.relation.ispartofANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
dc.subjectCLONING
dc.subjectCOELICOLOR A3(2)
dc.subjectESCHERICHIA-COLI
dc.subjectEXPRESSION
dc.subjectFAMILY-19 CHITINASES
dc.subjectfibronectin type III
dc.subjectGLYCOSYL HYDROLASES
dc.subjectMicrobiology
dc.subjectmultiple domain structure
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectOLIVACEOVIRIDIS
dc.subjectphylogeny
dc.subjectstreptomycetes
dc.subjectTHERMOSTABLE CHITINASE
dc.subjectTHERMOVIOLACEUS OPC-520
dc.titleDistribution and evolution of chitinase genes in Streptomyces species: involvement of gene-duplication and domain-deletion
dc.typejournal article
dc.identifier.doi10.1023/A:1024463113606
dc.identifier.isiISI:000183728500002
dc.description.volume84
dc.description.issue1
dc.description.startpage7
dc.description.endpage15
dc.publisher.placeVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
dcterms.isPartOf.abbreviationAntonie Van Leeuwenhoek
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