1-deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Seela, F | |
dc.contributor.author | Debelak, H | |
dc.contributor.author | Usman, N | |
dc.contributor.author | Burgin, A | |
dc.contributor.author | Beigelman, L | |
dc.date.accessioned | 2021-12-23T16:16:22Z | - |
dc.date.available | 2021-12-23T16:16:22Z | - |
dc.date.issued | 1998 | |
dc.identifier.issn | 03051048 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/11839 | - |
dc.description.abstract | The incorporation of 1-deazaadenosine (c(1)A, 1b) into a hammerhead ribozyme and the resulting catalytic activity is described. For this purpose the phosphoramidite 2a and the 3'-phosphonate 2b as well as Fractosil-linked 1-deazaadenosine (3b) were prepared. The methoxyacetyl group was used for the 6-amino group protection and the triisopropylsilyl residue was introduced as the 2'-OH protecting group. Replacement of residues A(14) and A(15.1) Of the hammerhead ribozyme by 1-deazaadenosine resulted in a significantly reduced catalytic activity. Substitution of the A(6), A(9) and A(13) residues has only a minor influence. The findings observed on ribozymes modified with 1-deazaadenosine were compared with those containing other adenosine analogues. | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS | |
dc.relation.ispartof | NUCLEIC ACIDS RESEARCH | |
dc.subject | 1-DEAZA-2'-DEOXYADENOSINE | |
dc.subject | AMINO-GROUPS | |
dc.subject | BINDING | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | CATALYTIC CLEAVAGE | |
dc.subject | CHEMICAL SYNTHESIS | |
dc.subject | CRYSTAL-STRUCTURE | |
dc.subject | DEPROTECTION | |
dc.subject | EFFICIENT CLEAVAGE | |
dc.subject | HOOGSTEEN-DUPLEX DNA | |
dc.subject | RNA | |
dc.title | 1-deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes | |
dc.type | journal article | |
dc.identifier.doi | 10.1093/nar/26.4.1010 | |
dc.identifier.isi | ISI:000072236300020 | |
dc.description.volume | 26 | |
dc.description.issue | 4 | |
dc.description.startpage | 1010 | |
dc.description.endpage | 1018 | |
dc.identifier.eissn | 13624962 | |
dc.publisher.place | GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND | |
dcterms.isPartOf.abbreviation | Nucleic Acids Res. | |
dcterms.oaStatus | Bronze, Green Published |
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geprüft am 18.05.2024