DC Field | Value | Language |
dc.contributor.author | Fuellbrunn, Nadia | |
dc.contributor.author | Li, Zehao | |
dc.contributor.author | Jorde, Lara | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Kurre, Rainer | |
dc.contributor.author | Langemeyer, Lars | |
dc.contributor.author | Yu, Changyuan | |
dc.contributor.author | Meyer, Carola | |
dc.contributor.author | Enderlein, Jorg | |
dc.contributor.author | Ungermann, Christian | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | You, Changjiang | |
dc.date.accessioned | 2021-12-23T16:16:35Z | - |
dc.date.available | 2021-12-23T16:16:35Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 2050084X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/11943 | - |
dc.description.abstract | Insights into the conformational organization and dynamics of proteins complexes at membranes is essential for our mechanistic understanding of numerous key biological processes. Here, we introduce graphene-induced energy transfer (GIET) to probe axial orientation of arrested macromolecules at lipid monolayers. Based on a calibrated distance-dependent efficiency within a dynamic range of 25 nm, we analyzed the conformational organization of proteins and complexes involved in tethering and fusion at the lysosome-like yeast vacuole. We observed that the membrane-anchored Rab7-like GTPase Ypt7 shows conformational reorganization upon interactions with effector proteins. Ensemble and time-resolved single-molecule GIET experiments revealed that the HOPS tethering complex, when recruited via Ypt7 to membranes, is dynamically alternating between a `closed' and an `open' conformation, with the latter possibly interacting with incoming vesicles. Our work highlights GIET as a unique spectroscopic ruler to reveal the axial orientation and dynamics of macromolecular complexes at biological membranes with subnanometer resolution. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [INST 190/146-3 (SFB944-P8), EXC 2067/1-390729940, INST 190/182-1, INST 190/149-3 (SFB944P11), INST190/152-3 (SFB944-Z), UN 111/12-1, YO 166/1-1]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870976]; Osnabrueck University; Deutsche Forschungsgemeinschaft INST 190/146-3 (SFB944-P8) Jacob Piehler; National Natural Science Foundation of China 31870976 Zehao Li Changyuan Yu; Intramural funding from Osnabrueck University Integrated Science Carola Meyer Christian Ungermann Jacob Piehler Changjiang You; Deutsche Forschungsgemeinschaft EXC 2067/1-390729940 Jorg Enderlein; Deutsche Forschungsgemeinschaft INST 190/182-1 Rainer Kurre Jacob Piehler; Deutsche Forschungsgemeinschaft INST 190/149-3 (SFB944P11) Christian Ungermann; Deutsche Forschungsgemeinschaft INST190/152-3 (SFB944-Z) Jacob Piehler; Deutsche Forschungsgemeinschaft UN 111/12-1 Christian Ungermann; Deutsche Forschungsgemeinschaft YO 166/1-1 Changjiang You; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | |
dc.language.iso | en | |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | |
dc.relation.ispartof | ELIFE | |
dc.subject | BINDING | |
dc.subject | Biology | |
dc.subject | DNA | |
dc.subject | FUSION | |
dc.subject | HOPS | |
dc.subject | IDENTIFICATION | |
dc.subject | Life Sciences & Biomedicine - Other Topics | |
dc.subject | LOCALIZATION | |
dc.subject | MICROSCOPY | |
dc.subject | ORIENTATION | |
dc.subject | RAB GTPASE | |
dc.subject | RECEPTOR INTERACTIONS | |
dc.title | Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer | |
dc.type | journal article | |
dc.identifier.doi | 10.7554/eLife.62501 | |
dc.identifier.isi | ISI:000614370500001 | |
dc.description.volume | 10 | |
dc.contributor.orcid | 0000-0001-5091-7157 | |
dc.contributor.orcid | 0000-0002-4309-0910 | |
dc.contributor.orcid | 0000-0003-0851-2767 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.researcherid | I-1723-2012 | |
dc.publisher.place | SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND | |
dcterms.isPartOf.abbreviation | eLife | |
dcterms.oaStatus | Green Published, gold | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0002-6872-6567 | - |
crisitem.author.orcid | 0000-0003-0851-2767 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | KuRa617 | - |
crisitem.author.netid | MeCa197 | - |
crisitem.author.netid | EnJo001 | - |
crisitem.author.netid | UnCh999 | - |
crisitem.author.netid | PiJa938 | - |
crisitem.author.netid | YoCh745 | - |