In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP
| Autor(en): | Kucher, Svetlana Korneev, Sergej Klare, Johann P. Klose, Daniel Steinhoff, Heinz-Jurgen |
Stichwörter: | AZIDES; Chemistry; Chemistry, Physical; CLICK CHEMISTRY; CYCLOADDITION; DISTANCE MEASUREMENTS; GD(III) COMPLEXES; IN-VITRO; Physics; Physics, Atomic, Molecular & Chemical; PROTEIN CONFORMATION; REDUCTION; STRATEGIES | Erscheinungsdatum: | 2020 | Herausgeber: | ROYAL SOC CHEMISTRY | Enthalten in: | PHYSICAL CHEMISTRY CHEMICAL PHYSICS | Band: | 22 | Ausgabe: | 24 | Startseite: | 13358 | Seitenende: | 13362 | Zusammenfassung: | Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures. |
ISSN: | 14639076 | DOI: | 10.1039/d0cp01930e |
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