Evaluation of Salmonella enterica Type III Secretion System Effector Proteins as Carriers for Heterologous Vaccine Antigens

Autor(en): Hegazy, Wael Abdel Halim
Xu, Xin
Metelitsa, Leonid
Hensel, Michael 
Stichwörter: DELIVERY; HOST-CELLS; IMMUNE-RESPONSES; Immunology; Infectious Diseases; RECOMBINANT VACCINES; STRATEGY; TRANSPORT
Erscheinungsdatum: 2012
Herausgeber: AMER SOC MICROBIOLOGY
Enthalten in: INFECTION AND IMMUNITY
Band: 80
Ausgabe: 3
Startseite: 1193
Seitenende: 1202
Zusammenfassung: 
Live attenuated strains of Salmonella enterica have a high potential as carriers of recombinant vaccines. The type III secretion system (T3SS)-dependent translocation of S. enterica can be deployed for delivery of heterologous antigens to antigen-presenting cells. Here we investigated the efficacy of various effector proteins of the Salmonella pathogenicity island (SPI2)-encoded T3SS for the translocation of model antigens and elicitation of immune responses. The SPI2 T3SS effector proteins SifA, SteC, SseL, SseJ, and SseF share an endosomal membrane-associated subcellular localization after translocation. We observed that all effector proteins could be used to translocate fusion proteins with the model antigens ovalbumin and listeriolysin into the cytosol of host cells. Under in vitro conditions, fusion proteins with SseJ and SteC stimulated T-cell responses that were superior to those triggered by fusion proteins with SseF. However, in mice vaccinated with Salmonella carrier strains, only fusion proteins based on SseJ or SifA elicited potent T-cell responses. These data demonstrate that the selection of an optimal SPI2 effector protein for T3SS-mediated translocation is a critical parameter for the rational design of effective Salmonella-based recombinant vaccines.
ISSN: 00199567
DOI: 10.1128/IAI.06056-11

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