The multigene family of the tobacco hornworm V-ATPase: novel subunits A, C, D, H, and putative isoforms

DC FieldValueLanguage
dc.contributor.authorMerzendorfer, H
dc.contributor.authorReineke, S
dc.contributor.authorZhao, XF
dc.contributor.authorJacobmeier, B
dc.contributor.authorHarvey, WR
dc.contributor.authorWieczorek, H
dc.date.accessioned2021-12-23T16:17:12Z-
dc.date.available2021-12-23T16:17:12Z-
dc.date.issued2000
dc.identifier.issn00052736
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/12251-
dc.description.abstractThe plasma membrane V-ATPase from Manduca sexta (Lepidoptera, Sphingidae) larval midgut is composed of at least 12 subunits, eight of which have already been identified molecularly [Wieczorek et al., J. Bioenerg. Biomembr. 31 (1999) 67-74]. Here we report primary sequences of subunits C, D, H and a, which previously had not been identified in insects. Expression of recombinant proteins, immunostaining and protein sequencing demonstrated that the corresponding proteins are subunits of the Manduca V-ATPase. Genomic Southern blot analysis indicated the existence of multiple genes encoding subunits G, a, c, d and e. Moreover. multiple transcripts were detected in Northern blots from midgut poly(A) RNA for subunits B, G, c and d. Thus, these polypeptides appear to exist as multiple isoforms that could be expressed either in different tissues or at distinct locations within a cell. By contrast subunits A, C, D, E, F and Fl appear to be encoded by single transcripts and therefore should be present in any Manduca V-ATPase, independent of its subcellular or cell specific origin. (C) 2000 Elsevier Science B.V. All rights reserved.
dc.description.sponsorshipNIAID NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI 22444] Funding Source: Medline; NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI022444] Funding Source: NIH RePORTER
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
dc.subjectANIMAL PLASMA-MEMBRANE
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCLONING
dc.subjectELECTRON-MICROSCOPY
dc.subjectisoform
dc.subjectManduca sexta
dc.subjectMANDUCA-SEXTA MIDGUT
dc.subjectmidgut
dc.subjectMOLECULAR CHARACTERIZATION
dc.subjectmultigene family
dc.subjectPOLYMERASE CHAIN-REACTION
dc.subjectPROTON-TRANSLOCATING ATPASE
dc.subjectPUMP
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectV-ATPase
dc.subjectvacuolar H+-translocating ATPase
dc.subjectVACUOLAR-TYPE ATPASE
dc.titleThe multigene family of the tobacco hornworm V-ATPase: novel subunits A, C, D, H, and putative isoforms
dc.typejournal article
dc.identifier.doi10.1016/S0005-2736(00)00233-9
dc.identifier.isiISI:000089222700010
dc.description.volume1467
dc.description.issue2
dc.description.startpage369
dc.description.endpage379
dc.contributor.orcid0000-0003-1809-4730
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationBiochim. Biophys. Acta-Biomembr.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidMeHa731-
crisitem.author.netidWiHe990-
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