The multigene family of the tobacco hornworm V-ATPase: novel subunits A, C, D, H, and putative isoforms
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Merzendorfer, H | |
dc.contributor.author | Reineke, S | |
dc.contributor.author | Zhao, XF | |
dc.contributor.author | Jacobmeier, B | |
dc.contributor.author | Harvey, WR | |
dc.contributor.author | Wieczorek, H | |
dc.date.accessioned | 2021-12-23T16:17:12Z | - |
dc.date.available | 2021-12-23T16:17:12Z | - |
dc.date.issued | 2000 | |
dc.identifier.issn | 00052736 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/12251 | - |
dc.description.abstract | The plasma membrane V-ATPase from Manduca sexta (Lepidoptera, Sphingidae) larval midgut is composed of at least 12 subunits, eight of which have already been identified molecularly [Wieczorek et al., J. Bioenerg. Biomembr. 31 (1999) 67-74]. Here we report primary sequences of subunits C, D, H and a, which previously had not been identified in insects. Expression of recombinant proteins, immunostaining and protein sequencing demonstrated that the corresponding proteins are subunits of the Manduca V-ATPase. Genomic Southern blot analysis indicated the existence of multiple genes encoding subunits G, a, c, d and e. Moreover. multiple transcripts were detected in Northern blots from midgut poly(A) RNA for subunits B, G, c and d. Thus, these polypeptides appear to exist as multiple isoforms that could be expressed either in different tissues or at distinct locations within a cell. By contrast subunits A, C, D, E, F and Fl appear to be encoded by single transcripts and therefore should be present in any Manduca V-ATPase, independent of its subcellular or cell specific origin. (C) 2000 Elsevier Science B.V. All rights reserved. | |
dc.description.sponsorship | NIAID NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI 22444] Funding Source: Medline; NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI022444] Funding Source: NIH RePORTER | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | |
dc.subject | ANIMAL PLASMA-MEMBRANE | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biophysics | |
dc.subject | CLONING | |
dc.subject | ELECTRON-MICROSCOPY | |
dc.subject | isoform | |
dc.subject | Manduca sexta | |
dc.subject | MANDUCA-SEXTA MIDGUT | |
dc.subject | midgut | |
dc.subject | MOLECULAR CHARACTERIZATION | |
dc.subject | multigene family | |
dc.subject | POLYMERASE CHAIN-REACTION | |
dc.subject | PROTON-TRANSLOCATING ATPASE | |
dc.subject | PUMP | |
dc.subject | SACCHAROMYCES-CEREVISIAE | |
dc.subject | V-ATPase | |
dc.subject | vacuolar H+-translocating ATPase | |
dc.subject | VACUOLAR-TYPE ATPASE | |
dc.title | The multigene family of the tobacco hornworm V-ATPase: novel subunits A, C, D, H, and putative isoforms | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/S0005-2736(00)00233-9 | |
dc.identifier.isi | ISI:000089222700010 | |
dc.description.volume | 1467 | |
dc.description.issue | 2 | |
dc.description.startpage | 369 | |
dc.description.endpage | 379 | |
dc.contributor.orcid | 0000-0003-1809-4730 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | Biochim. Biophys. Acta-Biomembr. | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | MeHa731 | - |
crisitem.author.netid | WiHe990 | - |
Seitenaufrufe
5
Letzte Woche
0
0
Letzter Monat
1
1
geprüft am 07.06.2024