Identification of Mature Atherosclerotic Plaque Proteome Signatures Using Data-Independent Acquisition Mass Spectrometry

Autor(en): Hansmeier, Nicole
Buttigieg, Josef
Kumar, Pankaj
Pelle, Shaneen
Choi, Kyoo Yoon
Kopriva, David
Chao, Tzu-Chiao
Stichwörter: A-IV; ABSOLUTE QUANTIFICATION; Biochemical Research Methods; Biochemistry & Molecular Biology; BIOMARKER; extracellular matrix; EXTRACELLULAR-MATRIX; MACROPHAGE-LIKE CELLS; plaque proteome; PROLIFERATION; PROTEINS; SHEAR-STRESS; smooth muscle cells; SMOOTH-MUSCLE-CELLS; vascular disease; VULNERABLE PLAQUE
Erscheinungsdatum: 2018
Volumen: 17
Ausgabe: 1
Startseite: 164
Seitenende: 176
Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. To gain deeper insights into the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention. Using a quantitative, data-independent proteome approach, we identified 4181 proteins with an average protein coverage of 45%. An analysis of the quantitative composition of the tissue revealed key players of vascular remodeling processes. Moreover, compared with proximal arterial tissue, 20 proteins in mature plaques were enriched, whereas 52 proteins were found in lower quantities. Among the proteins with increased abundance were prominent extracellular matrix proteins such as biglycan and lumican, whereas cytoskeletal markers (SMCs) were decreased. Taken together, this study provides the most comprehensive quantitative assessment of mature human plaque tissue to date, which indicates a central role of SMCs in the structure of advanced atherosclerotic plaques.
ISSN: 15353893
DOI: 10.1021/acs.jproteome.7b00487

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