DC Field | Value | Language |
dc.contributor.author | Eisele, Nico B. | |
dc.contributor.author | Frey, Steffen | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Goerlich, Dirk | |
dc.contributor.author | Richter, Ralf P. | |
dc.date.accessioned | 2021-12-23T16:17:32Z | - |
dc.date.available | 2021-12-23T16:17:32Z | - |
dc.date.issued | 2010 | |
dc.identifier.issn | 1469221X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/12307 | - |
dc.description.abstract | Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine-glycine (FG)-rich repeats (FG repeat domains), in a way that is at present poorly understood. We have developed ultrathin FG domain films that reproduce the mode of attachment and the density of FG repeats in NPCs, and that exhibit a thickness that corresponds to the nanoscopic dimensions of the native permeability barrier. By using a combination of biophysical characterization techniques, we quantified the binding of nuclear transport receptors (NTRs) to such FG domain films and analysed how this binding affects the swelling behaviour and mechanical properties of the films. The results extend our understanding of the interaction of FG domain assemblies with NTRs and contribute important information to refine the model of transport across the permeability barrier. | |
dc.description.sponsorship | Department of Industry of the Basque Government; Max Planck SocietyMax Planck Society; Deutscher Akademischer Austausch DienstDeutscher Akademischer Austausch Dienst (DAAD); This study was supported financially by funds from the Department of Industry of the Basque Government (to N.E. and R.P.R.), the Max Planck Society, and Deutscher Akademischer Austausch Dienst (to N.E.). | |
dc.language.iso | en | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.relation.ispartof | EMBO REPORTS | |
dc.subject | AFFINITY | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Cell Biology | |
dc.subject | DYNAMICS | |
dc.subject | FG repeat domain | |
dc.subject | GRADIENT | |
dc.subject | IMPORTIN-BETA | |
dc.subject | nuclear pore complex | |
dc.subject | nuclear transport receptor | |
dc.subject | nucleoporins | |
dc.subject | PERMEABILITY BARRIER | |
dc.subject | PORE COMPLEX | |
dc.subject | REGIONS | |
dc.subject | TRANSLOCATION | |
dc.title | Ultrathin nucleoporin phenylalanine-glycine repeat films and their interaction with nuclear transport receptors | |
dc.type | journal article | |
dc.identifier.doi | 10.1038/embor.2010.34 | |
dc.identifier.isi | ISI:000277157900013 | |
dc.description.volume | 11 | |
dc.description.issue | 5 | |
dc.description.startpage | 366 | |
dc.description.endpage | 372 | |
dc.contributor.orcid | 0000-0002-4343-5210 | |
dc.contributor.orcid | 0000-0003-3071-2837 | |
dc.contributor.orcid | 0000-0002-5134-9961 | |
dc.contributor.orcid | 0000-0001-7690-0660 | |
dc.contributor.researcherid | B-8296-2017 | |
dc.contributor.researcherid | B-3343-2013 | |
dc.contributor.researcherid | J-9136-2014 | |
dc.publisher.place | MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND | |
dcterms.isPartOf.abbreviation | EMBO Rep. | |
dcterms.oaStatus | hybrid, Green Published | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |