Molecular architecture of Manduca sexta midgut V-1 ATPase visualized by electron microscopy

DC FieldValueLanguage
dc.contributor.authorRadermacher, M
dc.contributor.authorRuiz, T
dc.contributor.authorHarvey, WR
dc.contributor.authorWieczorek, H
dc.contributor.authorGruber, G
dc.date.accessioned2021-12-23T16:17:46Z-
dc.date.available2021-12-23T16:17:46Z-
dc.date.issued1999
dc.identifier.issn00145793
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/12396-
dc.description.abstractThe structure of the V-1 ATPase from the tobacco hornworm Manduca sexta has been determined from electron micrographs of isolated, negatively stained specimens. The resulting images clearly show a pseudohexagonal arrangement of six equal-sized protein densities, presumably representing the three copies each of subunits A and B, which comprise the headpiece of the enzyme. A seventh density could be observed either centrally or asymmetrically to the hexamer, The maximum diameter of the V-1 complex in the hexagonal projection is 13 nm with each of the six peripheral densities being 3-4 nm in diameter. (C) 1999 Federation of European Biochemical Societies.
dc.description.sponsorshipPHS HHSUnited States Department of Health & Human ServicesUnited States Public Health Service [A1 22444] Funding Source: Medline
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofFEBS LETTERS
dc.subjectBiochemistry & Molecular Biology
dc.subjectBIOLOGICAL MACROMOLECULES
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectelectron microscopy
dc.subjectESCHERICHIA-COLI
dc.subjectimage processing
dc.subjectIMAGES
dc.subjectManduca sexta
dc.subjectMEMBRANE
dc.subjectPARTICLES
dc.subjectPURIFICATION
dc.subjectSUBUNIT
dc.subjectTOBACCO HORNWORM MIDGUT
dc.subjectV-1 ATPase
dc.subjectVACUOLAR-TYPE ATPASE
dc.titleMolecular architecture of Manduca sexta midgut V-1 ATPase visualized by electron microscopy
dc.typejournal article
dc.identifier.doi10.1016/S0014-5793(99)00739-5
dc.identifier.isiISI:000081242100030
dc.description.volume453
dc.description.issue3
dc.description.startpage383
dc.description.endpage386
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationFEBS Lett.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWiHe990-
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