Proton leakage across lipid bilayers: Oxygen atoms of phospholipid ester linkers align water molecules into transmembrane water wires
DC Element | Wert | Sprache |
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dc.contributor.author | Bozdaganyan, Marine E. | |
dc.contributor.author | Lokhmatikov, Alexey V. | |
dc.contributor.author | Voskoboynikova, Natalia | |
dc.contributor.author | Cherepanov, Dmitry A. | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.contributor.author | Shaitan, Konstantin V. | |
dc.contributor.author | Mulkidjanian, Armen Y. | |
dc.date.accessioned | 2021-12-23T16:18:20Z | - |
dc.date.available | 2021-12-23T16:18:20Z | - |
dc.date.issued | 2019 | |
dc.identifier.issn | 00052728 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/12637 | - |
dc.description.abstract | Up to half of the cellular energy gets lost owing to membrane proton leakage. The permeability of lipid bilayers to protons is by several orders of magnitude higher than to other cations, which implies efficient proton-specific passages. The nature of these passages remains obscure. By combining experimental measurements of proton flow across phosphatidylcholine vesicles, steered molecular dynamics (MD) simulations of phosphatidylcholine bilayers and kinetic modelling, we have analyzed whether protons could pass between opposite phospholipid molecules when they sporadically converge. The MD simulations showed that each time, when the phosphorus atoms of the two phosphatidylcholine molecules got closer than 1.6 nm, the eight oxygen atoms of their ester linkages could form a transmembrane `oxygen passage' along which several water molecules aligned into a water wire. Proton permeability along such water wires would be limited by rearrangement of oxygen atoms, which could explain the experimentally shown independence of the proton permeability of pH, H2O/D2O substitution, and membrane dipole potential. We suggest that protons can cross lipid bilayers by moving along short, selfsustaining water wires supported by oxygen atoms of lipid ester linkages. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG); German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD); EvoCell Program of the Osnabrueck University; Development Programme of the Lomonosov Moscow State University; Russia Government [AAAA-A19-119012890064-7]; Russian Science FoundationRussian Science Foundation (RSF) [14-50-00029, 14-14-00592, 17-14-01314]; We gratefully acknowledge helpful discussions with Drs Boris V. Chernyak, Michael Y. Galperin, Joachim Heberle, Gerhard Hummer, Elena A. Kotova, and Vladimir. P. Skulachev. This work was supported by the Deutsche Forschungsgemeinschaft, the German Academic Exchange Service (DAAD), the EvoCell Program of the Osnabrueck University, the Development Programme of the Lomonosov Moscow State University (supercomputers `Chebyshev' and `Lomonosov'), the Russia Government contract (AAAA-A19-119012890064-7), and grants from the Russian Science Foundation (14-50-00029, analysis of the MD data; 14-14-00592, kinetic modelling; 17-14-01314 mechanism of proton leakage in mitochondria). | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | |
dc.subject | ACID-BASE TITRATION | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Bioenergetics | |
dc.subject | Biophysics | |
dc.subject | Chemiosmotic coupling | |
dc.subject | DYNAMICS | |
dc.subject | ELECTROSTATIC INTERACTIONS | |
dc.subject | Ester lipids | |
dc.subject | Ether lipids | |
dc.subject | FREE-ENERGY | |
dc.subject | ION-PERMEABILITY | |
dc.subject | Lipid ester linkers | |
dc.subject | MEMBRANES | |
dc.subject | Mitochondria | |
dc.subject | MITOCHONDRIAL PROTON | |
dc.subject | MONTE-CARLO | |
dc.subject | PERMEATION | |
dc.subject | Proton permeability | |
dc.subject | Proton potential | |
dc.subject | TRANSPORT | |
dc.title | Proton leakage across lipid bilayers: Oxygen atoms of phospholipid ester linkers align water molecules into transmembrane water wires | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.bbabio.2019.03.001 | |
dc.identifier.isi | ISI:000471082200001 | |
dc.description.volume | 1860 | |
dc.description.issue | 6 | |
dc.description.startpage | 439 | |
dc.description.endpage | 451 | |
dc.contributor.orcid | 0000-0001-6286-4638 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.researcherid | ABH-3544-2020 | |
dc.contributor.researcherid | R-8391-2016 | |
dc.contributor.researcherid | ABF-2126-2020 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.contributor.researcherid | AAH-3608-2021 | |
dc.identifier.eissn | 18792650 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | Biochim. Biophys. Acta-Bioenerg. | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.orcid | 0000-0003-2317-0144 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | VoNa568 | - |
crisitem.author.netid | StHe633 | - |
crisitem.author.netid | ShKo001 | - |
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geprüft am 01.06.2024