Microarray-based detection of specific IgE against recombinant omega-5-gliadin in suspected wheat-dependent exercise-induced anaphylaxis
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Brans, Richard | |
dc.contributor.author | Sauer, Ilka | |
dc.contributor.author | Czaja, Katharina | |
dc.contributor.author | Pfuetzner, Wolfgang | |
dc.contributor.author | Merk, Hans F. | |
dc.date.accessioned | 2021-12-23T16:18:28Z | - |
dc.date.available | 2021-12-23T16:18:28Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 11671122 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/12700 | - |
dc.description.abstract | Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a rare IgE-mediated food allergy. Component-resolved measurement of specific IgE (sIgE) against omega-5-gliadin by fluorescence enzyme immunoassay (FEIA) has been postulated as a good predictive decision criterion in the diagnosis of WDEIA. More recently, microarray technology has been introduced into component-resolved diagnostics. The aim of this study was to evaluate the performance of an allergen microarray in the detection of sIgE against omega-5-gliadin in 10 patients with suspected WDEIA and high levels of sIgE against omega-5-gliadin (mean: 9.31 /- 7.53 kU/L, range: 4.24-25.8) as measured by FEIA. Using an old version of the microarray assay (ImmunoCAP ISACTM, Phadia), sIgE against omega-5gliadin was detected in only 3 of the first 6 patients. The same samples and those of another 4 patients were then analysed with an improved version of the microarray system, yielding elevated levels of sIgE against omega-5-gliadin in all patients. In conclusion, the old version of the microarray was not reliable for the detection of sIgE against omega-5-gliadin in the examined individuals with suspected WDEIA. In contrast, the improved version of the microarray seems to be as adequate as FEIA in the detection of sIgE against omega-5-gliadin. However, further large-scale studies are warranted to confirm these results. | |
dc.language.iso | en | |
dc.publisher | JOHN LIBBEY EUROTEXT LTD | |
dc.relation.ispartof | EUROPEAN JOURNAL OF DERMATOLOGY | |
dc.subject | allergen component | |
dc.subject | ALLERGEN-MICROARRAY | |
dc.subject | anaphylaxis | |
dc.subject | CHALLENGE | |
dc.subject | CHILDREN | |
dc.subject | Dermatology | |
dc.subject | DIAGNOSIS | |
dc.subject | gliadin | |
dc.subject | IgE | |
dc.subject | microarray | |
dc.subject | OMEGA-5 GLIADIN | |
dc.subject | TOOL | |
dc.subject | wheat allergy | |
dc.title | Microarray-based detection of specific IgE against recombinant omega-5-gliadin in suspected wheat-dependent exercise-induced anaphylaxis | |
dc.type | journal article | |
dc.identifier.doi | 10.1684/ejd.2012.1712 | |
dc.identifier.isi | ISI:000305692400009 | |
dc.description.volume | 22 | |
dc.description.issue | 3 | |
dc.description.startpage | 358 | |
dc.description.endpage | 362 | |
dc.identifier.eissn | 19524013 | |
dc.publisher.place | 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE | |
dcterms.isPartOf.abbreviation | Eur. J. Dermatol. | |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.orcid | 0000-0002-1245-024X | - |
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geprüft am 17.05.2024