OEP40, a Regulated Glucose-permeable -Barrel Solute Channel in the Chloroplast Outer Envelope Membrane

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dc.contributor.authorHarsman, Anke
dc.contributor.authorSchock, Annette
dc.contributor.authorHemmis, Birgit
dc.contributor.authorWahl, Vanessa
dc.contributor.authorJeshen, Ingrid
dc.contributor.authorBartsch, Philipp
dc.contributor.authorSchlereth, Armin
dc.contributor.authorPertl-Obermeyer, Heidi
dc.contributor.authorGoetze, Tom Alexander
dc.contributor.authorSoll, Juergen
dc.contributor.authorPhilippar, Katrin
dc.contributor.authorWagner, Richard
dc.date.accessioned2021-12-23T16:18:49Z-
dc.date.available2021-12-23T16:18:49Z-
dc.date.issued2016
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/12859-
dc.description.abstractChloroplasts and mitochondria are unique endosymbiotic cellular organelles surrounded by two membranes. Essential metabolic networking between these compartments and their hosting cells requires the exchange of a large number of biochemical pathway intermediates in a directed and coordinated fashion across their inner and outer envelope membranes. Here, we describe the identification and functional characterization of a highly specific, regulated solute channel in the outer envelope of chloroplasts, named OEP40. Loss of OEP40 function in Arabidopsis thaliana results in early flowering under cold temperature. The reconstituted recombinant OEP40 protein forms a high conductance -barrel ion channel with subconductant states in planar lipid bilayers. The OEP40 channel is slightly cation-selective PK+/PCl- approximate to 4:1 and rectifying (i?/i? 2) with a slope conductance of G(max) 690 picosiemens. The OEP40 channel has a restriction zone diameter of 1.4 nm and is permeable for glucose, glucose 1-phosphate and glucose 6-phosphate, but not for maltose. Moreover, channel properties are regulated by trehalose 6-phosphate, which cannot permeate. Altogether, our results indicate that OEP40 is a glucose-gate in the outer envelope membrane of chloroplasts, facilitating selective metabolite exchange between chloroplasts and the surrounding cell.
dc.description.sponsorshipHeisenberg Fellowship of the Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [PH73/6-1]; Supported by a Heisenberg Fellowship PH73/6-1 of the Deutsche Forschungsgemeinschaft.
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.relation.ispartofJOURNAL OF BIOLOGICAL CHEMISTRY
dc.subjectARABIDOPSIS
dc.subjectbeta-barrel protein
dc.subjectBiochemistry & Molecular Biology
dc.subjectchloroplast
dc.subjectCOLD-ACCLIMATION
dc.subjectDYNAMIC INTERFACE
dc.subjectglucose transport
dc.subjection channel
dc.subjectmembrane transport
dc.subjectMETABOLISM
dc.subjectmetabolite transport
dc.subjectouter membrane
dc.subjectPREDICTION
dc.subjectSECONDARY STRUCTURE
dc.subjectsolute channel
dc.subjectSTARCH DEGRADATION
dc.subjectTRANSCRIPTOME
dc.subjectTRANSPORTERS
dc.subjectTREHALOSE 6-PHOSPHATE
dc.titleOEP40, a Regulated Glucose-permeable -Barrel Solute Channel in the Chloroplast Outer Envelope Membrane
dc.typejournal article
dc.identifier.doi10.1074/jbc.M115.712398
dc.identifier.isiISI:000383241300030
dc.description.volume291
dc.description.issue34
dc.description.startpage17848
dc.description.endpage17860
dc.contributor.orcid0000-0001-9180-5995
dc.contributor.orcid0000-0002-2140-4907
dc.contributor.researcheridF-7942-2018
dc.contributor.researcheridI-5849-2015
dc.contributor.researcheridABC-3341-2020
dc.identifier.eissn1083351X
dc.publisher.place9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
dcterms.isPartOf.abbreviationJ. Biol. Chem.
dcterms.oaStatushybrid, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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