Solution Structure and Conformational Dynamics of Deoxyxylonucleic Acids (dXNA): An Orthogonal Nucleic Acid Candidate
DC Element | Wert | Sprache |
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dc.contributor.author | Maiti, Mohitosh | |
dc.contributor.author | Siegmund, Vanessa | |
dc.contributor.author | Abramov, Mikhail | |
dc.contributor.author | Lescrinier, Eveline | |
dc.contributor.author | Rosemeyer, Helmut | |
dc.contributor.author | Froeyen, Mathy | |
dc.contributor.author | Ramaswamy, Amutha | |
dc.contributor.author | Ceulemans, Arnout | |
dc.contributor.author | Marx, Andreas | |
dc.contributor.author | Herdewijn, Piet | |
dc.date.accessioned | 2021-12-23T16:18:54Z | - |
dc.date.available | 2021-12-23T16:18:54Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 09476539 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/12896 | - |
dc.description.abstract | Orthogonal nucleic acids are chemically modified nucleic acid polymers that are unable to transfer information with natural nucleic acids and thus can be used in synthetic biology to store and transfer genetic information independently. Recently, it was proposed that xylose-DNA (dXNA) can be considered to be a potential candidate for an orthogonal system. Herein, we present the structure in solution and conformational analysis of two self-complementary, fully modified dXNA oligonucleotides, as determined by CD and NMR spectroscopy. These studies are the initial experimental proof of the structural orthogonality of dXNAs. In aqueous solution, dXNA duplexes predominantly form a linear ladderlike (type-1) structure. This is the first example of a furanose nucleic acid that adopts a ladderlike structure. In the presence of salt, an equilibrium exists between two types of duplex form. The corresponding nucleoside triphosphates (dXNTPs) were synthesized and evaluated for their ability to be incorporated into a growing DNA chain by using several natural and mutant DNA polymerases. Despite the structural orthogonality of dXNA, DNA polymerase beta mutant is able to incorporate the dXNTPs, showing DNA-dependent dXNA polymerase activity. | |
dc.description.sponsorship | K. U. LeuvenKU Leuven; DFGGerman Research Foundation (DFG)European Commission; This work was financed by K. U. Leuven grants (IDO and GOA) and partially by the DFG. We are grateful to Dr. Koen Nauwelaerts for helpful discussions. We thank Prof. Arthur Van Aerschot and Guy Schepers for oligonucleotide synthesis and for providing UV melting data and Chantal Biernaux for editorial help. | |
dc.language.iso | en | |
dc.publisher | WILEY-V C H VERLAG GMBH | |
dc.relation.ispartof | CHEMISTRY-A EUROPEAN JOURNAL | |
dc.subject | AQUATICUS DNA-POLYMERASE | |
dc.subject | BUILDING-BLOCKS | |
dc.subject | CHEMICAL ETIOLOGY | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | DNA structures | |
dc.subject | helical structures | |
dc.subject | NMR spectroscopy | |
dc.subject | NMR-SPECTROSCOPY | |
dc.subject | nucleic acids | |
dc.subject | OLIGONUCLEOTIDES | |
dc.subject | orthogonal nucleic acids | |
dc.subject | REVERSE-TRANSCRIPTASE ACTIVITY | |
dc.subject | RNA | |
dc.subject | SOLID-PHASE SYNTHESIS | |
dc.subject | STABILITY | |
dc.subject | synthetic biology | |
dc.subject | XYLOSE-DNA | |
dc.title | Solution Structure and Conformational Dynamics of Deoxyxylonucleic Acids (dXNA): An Orthogonal Nucleic Acid Candidate | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/chem.201102509 | |
dc.identifier.isi | ISI:000298919400019 | |
dc.description.volume | 18 | |
dc.description.issue | 3 | |
dc.description.startpage | 869 | |
dc.description.endpage | 879 | |
dc.contributor.orcid | 0000-0002-8675-6540 | |
dc.contributor.orcid | 0000-0001-7066-4329 | |
dc.contributor.orcid | 0000-0002-4677-2263 | |
dc.contributor.orcid | 0000-0003-3589-8503 | |
dc.contributor.researcherid | I-1665-2014 | |
dc.contributor.researcherid | I-1511-2013 | |
dc.contributor.researcherid | G-9339-2016 | |
dc.publisher.place | PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY | |
dcterms.isPartOf.abbreviation | Chem.-Eur. J. | |
dcterms.oaStatus | Green Accepted, Green Submitted | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | RoHe783 | - |
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geprüft am 07.06.2024