A search for ceramide binding proteins using bifunctional lipid analogs yields CERT-related protein StarD7

Autor(en): Bockelmann, Svenja 
Mina, John G. M.
Korneev, Sergei 
Hassan, Dina G.
Mueller, Dagmar
Hilderink, Angelika
Vlieg, Hedwich C.
Raijmakers, Reinout
Heck, Albert J. R.
Haberkant, Per
Holthuis, Joost C. M.
Stichwörter: Biochemistry & Molecular Biology; CELLS; ceramide transfer protein; CHOLESTEROL; click chemistry; EADOCK DSS; IDENTIFICATION; INDUCED APOPTOSIS; lipid transfer protein; mitochondria; MITOCHONDRIAL OUTER-MEMBRANE; NONVESICULAR TRAFFICKING; phosphatidylcholine; PHOSPHATIDYLCHOLINE TRANSFER PROTEIN; photoaffinity labeling; steroidogenic acute regulatory protein D7; SWISS-MODEL; TRANSMEMBRANE DOMAIN
Erscheinungsdatum: 2018
Volumen: 59
Ausgabe: 3
Startseite: 515
Seitenende: 530
Ceramides are central intermediates of sphingolipid metabolism with dual roles as mediators of cellular stress signaling and mitochondrial apoptosis. How ceramides exert their cytotoxic effects is unclear and their poor solubility in water hampers a search for specific protein interaction partners. Here, we report the application of a photoactivatable and clickable ceramide analog, pacCer, to identify ceramide binding proteins and unravel the structural basis by which these proteins recognize ceramide. Besides capturing ceramide transfer protein (CERT) from a complex proteome, our approach yielded CERT-related steroidogenic acute regulatory protein D7 (StarD7) as novel ceramide binding protein. Previous work revealed that StarD7 is required for efficient mitochondrial import of phosphatidylcholine (PC) and serves a critical role in mitochondrial function and morphology. Combining site-directed mutagenesis and photoaffinity labeling experiments, we demonstrate that the steroidogenic acute regulatory transfer domain of StarD7 harbors a common binding site for PC and ceramide. While StarD7 lacks robust ceramide transfer activity in vitro, we find that its ability to shuttle PC between model membranes is specifically affected by ceramides. Besides demonstrating the suitability of pacCer as a tool to hunt for ceramide binding proteins, our data point at StarD7 as a candidate effector protein by which ceramides may exert part of their mitochondria- mediated cytotoxic effects.
ISSN: 00222275
DOI: 10.1194/jlr.M082354

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