Bacterial Porin Disrupts Mitochondrial Membrane Potential and Sensitizes Host Cells to Apoptosis
DC Element | Wert | Sprache |
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dc.contributor.author | Kozjak-Pavlovic, Vera | |
dc.contributor.author | Dian-Lothrop, Elke A. | |
dc.contributor.author | Meinecke, Michael | |
dc.contributor.author | Kepp, Oliver | |
dc.contributor.author | Ross, Katharina | |
dc.contributor.author | Rajalingam, Krishnaraj | |
dc.contributor.author | Harsman, Anke | |
dc.contributor.author | Hauf, Eva | |
dc.contributor.author | Brinkmann, Volker | |
dc.contributor.author | Guenther, Dirk | |
dc.contributor.author | Herrmann, Ines | |
dc.contributor.author | Hurwitz, Robert | |
dc.contributor.author | Rassow, Joachim | |
dc.contributor.author | Wagner, Richard | |
dc.contributor.author | Rudel, Thomas | |
dc.date.accessioned | 2021-12-23T16:19:16Z | - |
dc.date.available | 2021-12-23T16:19:16Z | - |
dc.date.issued | 2009 | |
dc.identifier.issn | 15537366 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13064 | - |
dc.description.abstract | The bacterial PorB porin, an ATP-binding beta-barrel protein of pathogenic Neisseria gonorrhoeae, triggers host cell apoptosis by an unknown mechanism. PorB is targeted to and imported by host cell mitochondria, causing the breakdown of the mitochondrial membrane potential (Delta psi(m)). Here, we show that PorB induces the condensation of the mitochondrial matrix and the loss of cristae structures, sensitizing cells to the induction of apoptosis via signaling pathways activated by BH3-only proteins. PorB is imported into mitochondria through the general translocase TOM but, unexpectedly, is not recognized by the SAM sorting machinery, usually required for the assembly of beta-barrel proteins in the mitochondrial outer membrane. PorB integrates into the mitochondrial inner membrane, leading to the breakdown of Delta psi(m). The PorB channel is regulated by nucleotides and an isogenic PorB mutant defective in ATP-binding failed to induce Delta psi(m) loss and apoptosis, demonstrating that dissipation of Delta psi(m) is a requirement for cell death caused by neisserial infection. | |
dc.description.sponsorship | Alexander von Humboldt FoundationAlexander von Humboldt Foundation; Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [SFB431, SPP1131]; BMBF through the RiNA-NetworkFederal Ministry of Education & Research (BMBF); This work was supported by a fellowship from the Alexander von Humboldt Foundation to V. K.-P. and by a grant from the Deutsche Forschungsgemeinschaft (DFG) SFB431 to R. W. and SPP1131 to J.R. and T. R., and the Senat of Berlin, and the BMBF through the RiNA-Network (http://www.rnanetwork. com). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.language.iso | en | |
dc.publisher | PUBLIC LIBRARY SCIENCE | |
dc.relation.ispartof | PLOS PATHOGENS | |
dc.subject | ASSEMBLY MACHINERY | |
dc.subject | BETA-BARREL PROTEINS | |
dc.subject | BIOGENESIS | |
dc.subject | CYTOCHROME-C RELEASE | |
dc.subject | EPITHELIAL-CELLS | |
dc.subject | IMPORT PORE | |
dc.subject | INNER MEMBRANE | |
dc.subject | Microbiology | |
dc.subject | NEISSERIA-GONORRHOEAE | |
dc.subject | OUTER-MEMBRANE | |
dc.subject | Parasitology | |
dc.subject | PROTECTS CELLS | |
dc.subject | Virology | |
dc.title | Bacterial Porin Disrupts Mitochondrial Membrane Potential and Sensitizes Host Cells to Apoptosis | |
dc.type | journal article | |
dc.identifier.doi | 10.1371/journal.ppat.1000629 | |
dc.identifier.isi | ISI:000272033300033 | |
dc.description.volume | 5 | |
dc.description.issue | 10 | |
dc.contributor.orcid | 0000-0002-6081-9558 | |
dc.contributor.orcid | 0000-0003-1414-6951 | |
dc.contributor.orcid | 0000-0001-9658-7018 | |
dc.contributor.researcherid | D-3081-2011 | |
dc.contributor.researcherid | N-2763-2017 | |
dc.contributor.researcherid | A-6015-2009 | |
dc.contributor.researcherid | C-4833-2013 | |
dc.contributor.researcherid | E-1494-2013 | |
dc.publisher.place | 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA | |
dcterms.isPartOf.abbreviation | PLoS Pathog. | |
dcterms.oaStatus | Green Submitted, Green Published, gold | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | WaRi703 | - |
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geprüft am 17.05.2024