GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis
DC Element | Wert | Sprache |
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dc.contributor.author | Wang, Shuoshuo | |
dc.contributor.author | Meyer, Heiko | |
dc.contributor.author | Ochoa-Espinosa, Amanda | |
dc.contributor.author | Buchwald, Ulf | |
dc.contributor.author | Oenel, Susanne | |
dc.contributor.author | Altenhein, Benjamin | |
dc.contributor.author | Heinisch, Juergen J. | |
dc.contributor.author | Affolter, Markus | |
dc.contributor.author | Paululat, Achim | |
dc.date.accessioned | 2021-12-23T16:19:27Z | - |
dc.date.available | 2021-12-23T16:19:27Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 00219533 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13147 | - |
dc.description.abstract | Here we report on the generation and in vivo analysis of a series of loss-of-function mutants for the Drosophila ArfGEF, Gartenzwerg. The Drosophila gene gartenzwerg (garz) encodes the orthologue of mammalian GBF1. garz is expressed ubiquitously in embryos with substantially higher abundance in cells forming diverse tubular structures such as salivary glands, trachea, proventriculus or hindgut. In the absence of functional Garz protein, the integrity of the Golgi complex is impaired. As a result, both vesicle transport of cargo proteins and directed apical membrane delivery are severely disrupted. Dysfunction of the Arf1-COPI machinery caused by a loss of Garz leads to perturbations in establishing a polarized epithelial architecture of tubular organs. Furthermore, insufficient apical transport of proteins and other membrane components causes incomplete luminal diameter expansion and deficiencies in extracellular matrix assembly. The fact that homologues of Garz are present in every annotated metazoan genome indicates that secretion processes mediated by the GBF-type ArfGEFs play a universal role in animal development. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 431, SFB 944]; SystemsX.ch; Swiss National Science FoundationSwiss National Science Foundation (SNSF)European Commission; Kantons of Basel-Stadt and Basel-Land; This work was supported by the Deutsche Forschungsgemeinschaft within the framework of the SFB 431 and SFB 944 to A.P. and to J.J.H. A.O. and M.A. were supported by SystemsX.ch within the framework of the WingX RID, the Swiss National Science Foundation and the Kantons of Basel-Stadt and Basel-Land. | |
dc.language.iso | en | |
dc.publisher | COMPANY BIOLOGISTS LTD | |
dc.relation.ispartof | JOURNAL OF CELL SCIENCE | |
dc.subject | ADP-RIBOSYLATION | |
dc.subject | ADP-ribosylation factor (Arf) | |
dc.subject | ARF | |
dc.subject | BINDING | |
dc.subject | Cell Biology | |
dc.subject | CIS-GOLGI | |
dc.subject | COPI | |
dc.subject | Extracellular matrix (ECM) | |
dc.subject | FACTORS GEA1P | |
dc.subject | GENES | |
dc.subject | Guanine nucleotide exchange factor (GEF) | |
dc.subject | Intracellular trafficking | |
dc.subject | Lipid homeostasis | |
dc.subject | NUCLEOTIDE EXCHANGE FACTORS | |
dc.subject | OF-FUNCTION SCREEN | |
dc.subject | PROTEIN SECRETION | |
dc.subject | Secretory pathway | |
dc.subject | TUBE MORPHOGENESIS | |
dc.subject | Tubulogenesis | |
dc.title | GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis | |
dc.type | journal article | |
dc.identifier.doi | 10.1242/jcs.092551 | |
dc.identifier.isi | ISI:000301551000020 | |
dc.description.volume | 125 | |
dc.description.issue | 2 | |
dc.description.startpage | 461 | |
dc.description.endpage | 472 | |
dc.contributor.orcid | 0000-0002-8216-6164 | |
dc.contributor.orcid | 0000-0002-5171-0016 | |
dc.contributor.orcid | 0000-0002-3304-4523 | |
dc.contributor.orcid | 0000-0003-1943-790X | |
dc.contributor.orcid | 0000-0002-8845-6859 | |
dc.contributor.researcherid | G-3801-2017 | |
dc.contributor.researcherid | J-6649-2014 | |
dc.identifier.eissn | 14779137 | |
dc.publisher.place | BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND | |
dcterms.isPartOf.abbreviation | J. Cell Sci. | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-8845-6859 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PaAc947 | - |
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geprüft am 02.06.2024